Developmental and Hormonal Regulation of Leptin Receptor (Ob-R) Messenger Ribonucleic Acid Expression in Rat Testis
Manuel Tena-Sempere,Leonor Pinilla,Fu-Ping Zhang,LC Gonzalez,Ilpo Huhtaniemi,Felipe F. Casanueva,Carlos Dieguez,Enrique Aguilar +7 more
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TLDR
The results indicate that testicular Ob-R gene expression is developmentally regulated, imprinted by the neonatal endocrine milieu, and sensitive to regulation by leptin and gonadotropins.Abstract:
In target tissues, leptin receptor (Ob-R) gene expression results in an array of alternatively spliced isoforms (Ob-Ra to ObRf) with different functional features. Recent evidence has pointed to a direct role of leptin in the control of testicular function. However, complete elucidation of the pattern of Ob-R gene expression in the male gonad is still pending. The focus of this study was to characterize in detail the developmental pattern of expression and hormonal regulation of Ob-R gene in rat testis. To this end, the overall expression of Ob-R mRNA was compared to that of the fully functional, long Ob-Rb isoform in different experimental settings, using semiquantitative reverse transcription-polymerase chain reaction. Expression of Ob-R mRNA was detected in testes from 15-, 30-, 45-, and 75-day-old rats at rather constant relative levels. In contrast, testicular expression of Ob-Rb mRNA was higher in pubertal testes (15- to 30-day-old rats) and declined in adulthood. In testes from 30-day-old animals, analysis of isoform distribution revealed that, in addition to abundant Ob-Rb mRNA levels, expression of Ob-Ra, Ob-Rf, and, to a lesser extent, Ob-Rc and Ob-Re messages is detected. Testicular Ob-R mRNA expression appeared sensitive to neonatal imprinting as neonatal treatment with estradiol benzoate (500 mg/rat; Day 1 postpartum) resulted in a persistent increase (P , 0.01) in the relative expression level of Ob-R mRNA, a phenomenon only partially mimicked by neonatal suppression of serum gonadotropins by means of LHRH-antagonist administration. In addition, neonatal estrogenization differentially altered the pattern of expression of Ob-R isoforms in adult rat testis, as expression of Ob-Rb mRNA was decreased to undetectable levels, whereas that of Ob-Rc remained unaltered, and Ob-Ra, Ob-Rf, and, to a lesser extent, Ob-Re mRNA levels were significantly increased (P , 0.01) by neonatal exposure to estrogen. Finally, down-regulation of testicular Ob-R gene expression by homologous and heterologous signals was demonstrated as relative levels of Ob-R and Ob-Rb mRNAs were significantly decreased (P , 0.01), in a coordinate manner, in rat testis after exposure to human recombinant leptin in vitro, and after stimulation with hCG and FSH in vivo. In conclusion, our results indicate that testicular Ob-R gene expression is developmentally regulated, imprinted by the neonatal endocrine milieu, and senread more
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Novel Expression and Functional Role of Ghrelin in Rat Testis
Manuel Tena-Sempere,M.L. Barreiro,LC Gonzalez,F Gaytan,Fu-Ping Zhang,Jorge E. Caminos,Leonor Pinilla,Felipe F. Casanueva,Carlos Dieguez,Enrique Aguilar +9 more
TL;DR: The present results underscore an unexpected role of ghrelin as signal with ability to potentially modulate not only growth and body weight homeostasis but also reproductive function, a phenomenon also demonstrated recently for the adipocyte-derived hormone, leptin.
Journal ArticleDOI
Leptin in male reproduction: the testis paradigm.
TL;DR: Overall, the current data indicate that the testis is a direct target for leptin actions, and the available evidence is suggestive of a tightly regulated, complex mode of action of leptin at different levels of the male gonadal axis that involves not only stimulatory but also inhibitory effects.
Journal ArticleDOI
Leptin: a possible metabolic signal affecting reproduction.
TL;DR: Evidence to support direct action of leptin at the level of the gonad indicates that the leptin receptor and its mRNA are present in ovarian tissue of several species, including cattle, which may lead to a more definitive role of leptin in domestic animal reproduction.
Journal ArticleDOI
Cellular Location and Hormonal Regulation of Ghrelin Expression in Rat Testis
M.L. Barreiro,F Gaytan,Jorge E. Caminos,Leonor Pinilla,Felipe F. Casanueva,Enrique Aguilar,Carlos Dieguez,Manuel Tena-Sempere +7 more
TL;DR: Results demonstrate that mature Leydig cells are the source of ghrelin expression in rat testis, the protein being expressed in both fetal- and adult-type Leydigs cells, and indicate that testicular expression of gh Relin is hormonally regulated and is at least partially dependent on pituitary LH.
Journal ArticleDOI
Molecular mechanisms of leptin action in adult rat testis: potential targets for leptin-induced inhibition of steroidogenesis and pattern of leptin receptor messenger ribonucleic acid expression
Manuel Tena-Sempere,PR Manna,Fu-Ping Zhang,Leonor Pinilla,LC Gonzalez,C. Dieguez,Ilpo Huhtaniemi,Enrique Aguilar +7 more
TL;DR: Results indicate that decreased expression of mRNAs encoding several up-stream elements in the steroidogenic pathway may contribute, at least partially, to leptin-induced inhibition of testicular steroidogenesis.
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