Journal ArticleDOI
Diagnosis of Mycoplasma hyopneumoniae.
TLDR
The use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories as mentioned in this paper.Abstract:
Mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. This small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. Confirming the presence of M. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. While culture of the organism remains the gold standard for identification, the use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories. Because of the role M. hyopneumoniae plays in increasing the severity of pneumonia associated with concurrent bacterial and viral infections, understanding the pathogenesis and diagnostic assays available is critical for developing effective intervention strategies to control respiratory disease on a herd basis.read more
Citations
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Journal ArticleDOI
Repetitive Elements in Mycoplasma hyopneumoniae Transcriptional Regulation.
Amanda Malvessi Cattani,Franciele Maboni Siqueira,Rafael Lucas Muniz Guedes,Irene Silveira Schrank +3 more
TL;DR: Findings suggest a potential regulatory role of tandem and palindromic DNA repeats in the M. hyopneumoniae transcriptional profile, and comparative analysis between distinct repetitive sequences found in related mycoplasma genomes demonstrated different percentages of conservation among pathogenic and nonpathogenic strains.
Journal ArticleDOI
Status and gaps of research on respiratory disease pathogens of swine in Africa
Peter Oba,Peter Oba,Peter Oba,Barbara Wieland,Frank Norbert Mwiine,Joseph Erume,Elin Gertzell,Magdalena Jacobson,Michel M. Dione +8 more
TL;DR: The purpose of this review was to document the current status of research on five important respiratory pathogens of swine in Africa to inform future research and interventions and highlight knowledge and information gaps on epidemiologic aspects as well as economic impacts of the various pathogens reported in swineIn Africa.
Journal ArticleDOI
Pneumonia enzoótica em javalis (Sus scrofa)
TL;DR: The aim of this paper is to describe the clinical, epidemiological, pathological, bacteriological and immunohistochemical aspects of a pneumonia outbreak in a wild pig farm in the Distrito Federal, Brazil, which is the first report of bronchopneumonia in wild boars associated with M. hyop pneumoniae infection.
Journal ArticleDOI
Global analysis of sRNA target genes in Mycoplasma hyopneumoniae
TL;DR: The results indicate that bacterial sRNAs could regulate a number of targets with various outcomes, and different correlations between the levels of sRNA transcripts and their target gene mRNAs were found, which suggest that the regulation of gene expression via s RNAs may play an important role in mycoplasma.
Journal ArticleDOI
Performance of Commercial Mycoplasma hyopneumoniae Serum Enzyme-Linked Immunosorbent Assays under Experimental and Field Conditions.
Ana Paula Serafini Poeta Silva,Ronaldo Magtoto,Henrique Meiroz de Souza Almeida,Aric McDaniel,Precy D. Magtoto,Rachel J. Derscheid,Maria M. Merodio,Franco Matias Ferreyra,Igor Renan Honorato Gatto,David H. Baum,Maria J. Clavijo,Bailey Arruda,Jeffrey J. Zimmerman,Luis G. Giménez-Lirola +13 more
TL;DR: An assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance is provided.
References
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Book
Diseases of swine.
TL;DR: In this article, the characteristics of a variety of diseases of swine and methods for their prevention and treatment are described, as well as methods to detect and treat these diseases in swine.
Journal ArticleDOI
Mycoplasma hyopneumoniae Potentiation of Porcine Reproductive and Respiratory Syndrome Virus-Induced Pneumonia
Eileen L. Thacker,Patrick G. Halbur,Richard F. Ross,Roongroje Thanawongnuwech,Roongroje Thanawongnuwech,Brad J. Thacker +5 more
TL;DR: Results indicate that M. hyopneumoniae infection potentiates PRRSV-induced disease and lesions, which is important with respect to the control of respiratory disease in pigs and has implications in elucidating the potential contribution of mycoplasmas in the pathogenesis of viral infections of other species, including humans.
Journal Article
Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.
TL;DR: A description is given of a new medium with which primary isolation of mycoplasma species of the porcine respiratory tract is usually successful, and various additives often recommended for myCoplasma cultivation have been examined for growth promoting effect.
Journal ArticleDOI
Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures.
M C DeBey,Richard F. Ross +1 more
TL;DR: In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium.
Journal ArticleDOI
Mycoplasma hyopneumoniae infection in pigs; duration of the disease and evaluation of four diagnostic assays
Vibeke Sørensen,Peter Ahrens,Kristen Barfod,Anne Avlund Feenstra,Niels Christian Feld,N.F. Friis,Vivi Bille-Hansen,Niels Jensen,Michael W. Pedersen +8 more
TL;DR: An evaluation of cultivation, immunofluorescence, ELISA and polymerase chain reaction for demonstration of M. hyopneumoniae in lungs showed that all four methods have a high sensitivity in the acute stages of pneumonia.