Journal ArticleDOI
Diagnosis of Mycoplasma hyopneumoniae.
TLDR
The use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories as mentioned in this paper.Abstract:
Mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. This small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. Confirming the presence of M. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. While culture of the organism remains the gold standard for identification, the use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories. Because of the role M. hyopneumoniae plays in increasing the severity of pneumonia associated with concurrent bacterial and viral infections, understanding the pathogenesis and diagnostic assays available is critical for developing effective intervention strategies to control respiratory disease on a herd basis.read more
Citations
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Frequency of PRRS live vaccine virus (European and North American genotype) in vaccinated and non-vaccinated pigs submitted for respiratory tract diagnostics in North-Western Germany.
Elisabeth grosse Beilage,Heiko Nathues,D. Meemken,Timm C. Harder,Marcus G. Doherr,Inga Grotha,Irene Greiser-Wilke +6 more
TL;DR: The dynamics of NA genotype vaccine and EU wild-type virus corresponds with studies on PRRSV spread in endemically infected herds and the potential of spontaneous spread of the NA genotypes should be considered in the planning of eradication programs.
Journal ArticleDOI
Vaccination of piglets up to 1 week of age with a single-dose Mycoplasma hyopneumoniae vaccine induces protective immunity within 2 weeks against virulent challenge in the presence of maternally derived antibodies.
Stephen R. Wilson,Leen Van Brussel,Gillian Saunders,Paul Runnels,Lucas P. Taylor,Dan Fredrickson,Jeremy Salt +6 more
TL;DR: It is demonstrated that administration of an inactivated Mycoplasma hyopneumoniae vaccine to piglets less than 1 week old is efficacious under field conditions and reduces the level of lung lesions observed in comparison to that in control pigs.
Journal ArticleDOI
Mycoplasma non-coding RNA: identification of small RNAs and targets.
Franciele Maboni Siqueira,Guilherme Loss de Morais,Susan Higashi,Susan Higashi,Susan Higashi,Laura Scherer Beier,Gabriela Merker Breyer,Caio Padoan de Sá Godinho,Marie-France Sagot,Marie-France Sagot,Marie-France Sagot,Irene Silveira Schrank,Arnaldo Zaha,Ana Tereza Ribeiro de Vasconcelos +13 more
TL;DR: A detailed investigation revealed a differential expression profile for two M. hyopneumoniae sRNAs in response to oxidative and heat shock stress conditions, suggesting that their expression is influenced by environmental signals.
Journal ArticleDOI
Efficacy of combined porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae vaccination in piglets
C. S. Drexler,M. H. Witvliet,M. Raes,M. van de Laar,A. A. S. Eggen,E. L. Thacker,E. L. Thacker +6 more
TL;DR: There were no significant differences between the protective efficacy of the combined vaccine protocol and the protective effectiveness of the two single vaccines, indicating that PRRSV vaccination did not interfere with M hyopneumoniae vaccine efficacy and vice versa.
Journal ArticleDOI
Biofilm formation and determination of minimum biofilm eradication concentration of antibiotics in Mycoplasma hyopneumoniae
Dereje Damte Tassew,Abraham Fikru Mechesso,Na-Hye Park,Ju-Beom Song,Joo-Woon Shur,Seung-Chun Park +5 more
TL;DR: The results demonstrated an apparent line of biofilm growth in 3 of the strains isolated from swine with confirmed cases of enzootic pneumonia, the first report ofBiofilm formation in M. hyopneumoniae.
References
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Book
Diseases of swine.
TL;DR: In this article, the characteristics of a variety of diseases of swine and methods for their prevention and treatment are described, as well as methods to detect and treat these diseases in swine.
Journal ArticleDOI
Mycoplasma hyopneumoniae Potentiation of Porcine Reproductive and Respiratory Syndrome Virus-Induced Pneumonia
Eileen L. Thacker,Patrick G. Halbur,Richard F. Ross,Roongroje Thanawongnuwech,Roongroje Thanawongnuwech,Brad J. Thacker +5 more
TL;DR: Results indicate that M. hyopneumoniae infection potentiates PRRSV-induced disease and lesions, which is important with respect to the control of respiratory disease in pigs and has implications in elucidating the potential contribution of mycoplasmas in the pathogenesis of viral infections of other species, including humans.
Journal Article
Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey.
TL;DR: A description is given of a new medium with which primary isolation of mycoplasma species of the porcine respiratory tract is usually successful, and various additives often recommended for myCoplasma cultivation have been examined for growth promoting effect.
Journal ArticleDOI
Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures.
M C DeBey,Richard F. Ross +1 more
TL;DR: In vivo- and in vitro-grown Mycoplasma hyopneumoniae organisms were inoculated onto newborn piglet tracheal organ cultures to provide a model for interaction of this organism with ciliated respiratory epithelium.
Journal ArticleDOI
Mycoplasma hyopneumoniae infection in pigs; duration of the disease and evaluation of four diagnostic assays
Vibeke Sørensen,Peter Ahrens,Kristen Barfod,Anne Avlund Feenstra,Niels Christian Feld,N.F. Friis,Vivi Bille-Hansen,Niels Jensen,Michael W. Pedersen +8 more
TL;DR: An evaluation of cultivation, immunofluorescence, ELISA and polymerase chain reaction for demonstration of M. hyopneumoniae in lungs showed that all four methods have a high sensitivity in the acute stages of pneumonia.