Distinct cholecystokinin receptors in brain and pancreas.
TLDR
Both pancreas and brain receptors show greater sensitivity to sulfated than to desulfated COOH-terminal octapeptide of CCK and display dissociation constants of 0.3-9.5 nM, which indicate that brain and pancreAs possess distinct CCK receptors, the two tissues show some similarities.Abstract:
125I-Labeled (Bolton-Hunter) cholecystokinin triacontatriapeptide (CCK-33) binds saturably and reversibly to distinct receptors in brain and pancreatic membranes. The peptide specificity of pancreatic CCK binding is the same as that for pancreatic amylase release. In brain, gastrin and pentagastrin display nanomolar affinity for binding sites, whereas in pancreas these two peptides are virtually inactive. Though these differences indicate that brain and pancreas possess distinct CCK receptors, the two tissues show some similarities. Both pancreas and brain receptors show greater sensitivity to sulfated than to desulfated COOH-terminal octapeptide of CCK and display dissociation constants of 0.3-9.5 nM. The pancreas possesses about 300 times more binding sites than does brain. CCK binding in both brain and pancreas is enhanced by divalent cations and reduced by monovalent cations. Receptor binding in both tissues is regulated in a selective fashion by guanyl nucleotides.read more
Citations
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Neurotransmitter regulation of dopamine neurons in the ventral tegmental area.
TL;DR: Understanding the dynamic range of a transmitter's action and how this couples in concert with other transmitters to modulate dopamine neurons in the VTA is essential to defining the role of dopamine cells in the etiology and maintenance of neuropsychiatric disorders.
Journal ArticleDOI
Biochemical and pharmacological characterization of an extremely potent and selective nonpeptide cholecystokinin antagonist
TL;DR: L-364,718 exhibited a very high selectivity for peripheral CCK receptors relative to brain CCK, gastrin, and various other peptide and nonpeptide receptors in both in vitro radioligand and isolated tissue assays.
Journal ArticleDOI
Brain peptides as neurotransmitters.
TL;DR: Peptides, such as cholecystokinin and vasoactive intestinal polypeptide, were known as intestinal hormones and later recognized as brain constituents and may also be central neurotransmitters.
Journal ArticleDOI
Cholecystokinin and Gastrin Receptors
TL;DR: General properties of the receptors are described, such as the different signaling pathways used to exert short- and long-term effects and the structural data that explain their binding properties, activation, and regulation are described.
Journal Article
International Union of Pharmacology. XXI. Structure, distribution, and functions of cholecystokinin receptors.
Florence Noble,Stephen A. Wank,Jacqueline N. Crawley,Jacques Bradwejn,Kim B. Seroogy,M. Hamon,Bernard P. Roques +6 more
TL;DR: The peptide cholecystokinin has been shown to mediate pancreatic secretion and contraction of gallbladder and was described in the mammalian central nervous system (CNS) as a gastrin-like peptide.
References
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Journal ArticleDOI
Endogenous opioid peptides: multiple agonists and receptors
TL;DR: It is concluded that the opioid peptidergic system has agonists of different characteristics which interact with more than one type of receptor.
Journal ArticleDOI
Immunochemical studies on cholecystokinin. II. Distribution and molecular heterogeneity in the central nervous system and small intestine of man and hog.
TL;DR: Using sequence-specific radioimmunoassays, the distribution and heterogeneity of cholecystokinin (CCK) has been determined in extracts of tissues from the central nervous system and small intestine of adult man and hog.
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New peptide in the vertebrate CNS reacting with antigastrin antibodies.
TL;DR: Evidence for the presence in the vertebrate nervous system of a hitherto undescribed brain gastrin immunoassayable peptide (BGP) is presented here.
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Localization and molecular heterogeneity of cholecystokinin in the central and peripheral nervous system
LI Larsson,Jens F. Rehfeld +1 more
TL;DR: Immunocytochemistry and radioimmunochemistry demonstrate the occurrence of the gastrointestinal hormone cholecystokinin (CCK) in both the central and peripheral nervous system of the guinea pig.
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Immunochemical evidence of cholecystokinin-like peptides in brain
TL;DR: The present study found the distribution of the brain components on Sephadex G-25 to differ from those of previously characterised forms of gastrin and CCK, suggesting that the brain factors resembled CCK-like peptides more closely than gastrin- like peptides.