Effects of a Chinese Herbal Medicine, Guan-Jen-Huang (Aeginetia indica Linn.), on Renal Cancer Cell Growth and Metastasis.
Yu-Huei Liu,Meng-Luen Li,Meng-Yu Hsu,Ya-Yueh Pang,I-Ling Chen,Ching-Kuei Chen,Sai-Wen Tang,Hsuan-Yuan Lin,Jung-Yaw Lin +8 more
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TLDR
GJH extract has a synergistic effect on apoptosis induced by chemotherapeutic agents and an inhibitory effect on cell adhesion, migration, and invasion, providing evidence for the use of water-based extracts of GJH as novel alternative therapeutic agents in the treatment of human renal cancer.Abstract:
Aeginetia indica Linn. (Guan-Jen-Huang, GJH), a traditional Chinese herb, has the potential to be an immunomodulatory agent. The purpose of this study was to explore the effect of GJH in the treatment of renal cancer. Concentration-effect curves for the influence of GJH on cellular proliferation showed a biphasic shape. Besides, GJH had a synergistic effect on cytotoxicity when combined with 5-fluorouracil (5-FU)which may be due to the alternation of the chemotherapeutic agent resistance-related genes and due to the synergistic effects on apoptosis. In addition, treatment with GJH extract markedly reduced 786-O cell adherence to human umbilical vein endothelial cells (HUVECs) and decreased 786-O cell migration and invasion. In a xenograft animal model, GJH extract had an inhibitory effect on tumor cell-induced metastasis. Moreover, western blot analysis showed that the expression of intercellular adhesion molecule-1 (ICAM-1) in 786-O cells was significantly decreased by treatment with GJH extract through inactivation of nuclear factor-κB (NF-κB). These results suggest that GJH extract has a synergistic effect on apoptosis induced by chemotherapeutic agents and an inhibitory effect on cell adhesion, migration, and invasion, providing evidence for the use of water-based extracts of GJH as novel alternative therapeutic agents in the treatment of human renal cancer.read more
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References
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Seed extract of Aeginetia indica L induces cytokine production and lymphocyte proliferation in vitro
TL;DR: In vitro effects of AIL on various lymphoid cells found that CD4+ T cells were main producers of IL-2 and TNF upon the stimulation with ALL in vitro, while both CD4- and CD8- T cells secreted IFN and ALL was mitogenic in vitro to T enriched splenic lymphocytes as well as B enriching lymphocytes.
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Serum Levels of Soluble Cell Adhesion Molecules (ICAM-1, VCAM-1, E-Selectin) and of Cytokine TNF-α Increase during Interleukin-2 Therapy
Claudio Fortis,Laura Galli,Giuseppe Consogno,Giovanni Citterio,Paola Matteucci,Ugo Scaglietti,Eraldo O. Bucci +6 more
TL;DR: It is suggested that elevated levels of soluble adhesion molecules and TNF-alpha in the blood of IL-2-treated patients may arise from a systemic inflammatory reaction producing endothelial cell activation.
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Th1-cytokine induction and anti-tumor effect of 55 kDa protein isolated from Aeginetia indica L., a parasitic plant.
Go Ohe,Masato Okamoto,Tetsuya Oshikawa,Sachiko Furuichi,Hidetomo Nishikawa,Tomoyuki Tano,Kayo Uyama,Takashi Bando,Hideo Yoshida,Toru Sakai,Kunisuke Himeno,Mitsunobu Sato,Shinya Ohkubo +12 more
TL;DR: It is suggested that AILb-A is a potent Th1 inducer and may be a useful immunotherapeutic agent for patients with malignant diseases.
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Neoadjuvant and adjuvant strategies in renal cell carcinoma: more questions than answers.
TL;DR: Neoadjuvant treatment is another treatment modality currently being evaluated for patients with early disease and in patients with metastatic RCC with inoperable primary tumors.
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Dissociation between the mitogenic effect and antitumor activity of seed extract from Aeginetia indica L.
Jian-Guo Chai,Masato Okamoto,Takashi Bando,Hideyuki Nagasawa,Hajime Hisaeda,Tohru Sakai,Kunisuke Himeno,Mitsunobu Sato,Shinya Ohkubo +8 more
TL;DR: It is demonstrated that AIL contains polysaccharides and proteins that induced B cell mitogenic and thymocyte co-stimulatory effects in vitro, while the proteins, especially a 50-60 kDa protein containing non-carbohydrate epitopes recognized by the mAbs, mediated antitumor activity in vivo.