Effects of a Chinese Herbal Medicine, Guan-Jen-Huang (Aeginetia indica Linn.), on Renal Cancer Cell Growth and Metastasis.
Yu-Huei Liu,Meng-Luen Li,Meng-Yu Hsu,Ya-Yueh Pang,I-Ling Chen,Ching-Kuei Chen,Sai-Wen Tang,Hsuan-Yuan Lin,Jung-Yaw Lin +8 more
Reads0
Chats0
TLDR
GJH extract has a synergistic effect on apoptosis induced by chemotherapeutic agents and an inhibitory effect on cell adhesion, migration, and invasion, providing evidence for the use of water-based extracts of GJH as novel alternative therapeutic agents in the treatment of human renal cancer.Abstract:
Aeginetia indica Linn. (Guan-Jen-Huang, GJH), a traditional Chinese herb, has the potential to be an immunomodulatory agent. The purpose of this study was to explore the effect of GJH in the treatment of renal cancer. Concentration-effect curves for the influence of GJH on cellular proliferation showed a biphasic shape. Besides, GJH had a synergistic effect on cytotoxicity when combined with 5-fluorouracil (5-FU)which may be due to the alternation of the chemotherapeutic agent resistance-related genes and due to the synergistic effects on apoptosis. In addition, treatment with GJH extract markedly reduced 786-O cell adherence to human umbilical vein endothelial cells (HUVECs) and decreased 786-O cell migration and invasion. In a xenograft animal model, GJH extract had an inhibitory effect on tumor cell-induced metastasis. Moreover, western blot analysis showed that the expression of intercellular adhesion molecule-1 (ICAM-1) in 786-O cells was significantly decreased by treatment with GJH extract through inactivation of nuclear factor-κB (NF-κB). These results suggest that GJH extract has a synergistic effect on apoptosis induced by chemotherapeutic agents and an inhibitory effect on cell adhesion, migration, and invasion, providing evidence for the use of water-based extracts of GJH as novel alternative therapeutic agents in the treatment of human renal cancer.read more
Citations
More filters
Journal ArticleDOI
Oxymatrine inhibits the proliferation of prostate cancer cells in vitro and in vivo
TL;DR: The results may provide a novel approach for the development of prostate cancer therapy using oxymatrine, which is derived from the traditional Chinese herb, Sophora flavescens, via the regulation of apoptosis-associated gene expression.
Journal ArticleDOI
Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN
TL;DR: In vivo assay indicated that Scutellarin possessed anti-cancer effect on xenograft without triggering toxic effect, suggesting that ScUTellarin might serve as a potential therapeutic agent in RCC treatment.
Journal ArticleDOI
Celastrus orbiculatus extract induces mitochondrial-mediated apoptosis in human hepatocellular carcinoma cells.
Hua Zhang,Yayun Qian,Yanqing Liu,Guoqing Li,Pingfang Cui,Yaodong Zhu,Hui Ma,Xue Ji,Shiyu Guo,Hisamits Tadashi +9 more
TL;DR: COE induces mitochondrial-mediated, caspase-dependent apoptosis in HCCLM6 cells, which might be attributed to the activation of mitogen-activated protein kinase (MAPK) and inhibition of Akt signaling pathways, which suggest that COE may be a potential treatment for human hepatocellular carcinoma.
Journal ArticleDOI
Germacrone inhibits the proliferation of glioma cells by promoting apoptosis and inducing cell cycle arrest
TL;DR: It is suggested that germacrone may be a novel potent chemopreventive drug candidate for gliomas via regulating the expression of proteins associated with apoptosis and G1 cell cycle arrest.
Journal ArticleDOI
Reversion of P-Glycoprotein-Mediated Multidrug Resistance in Human Leukemic Cell Line by Diallyl Trisulfide
TL;DR: DATS can serve as a novel, nontoxic modulator of MDR, and can reverse the MDR of K562/A02 cells in vitro by increasing intracellular adriamycin concentration and inducing apoptosis, and it is proved for the first time that the suppression of NF-κB possibly involves the molecular mechanism in the course of reversion by DATS.
References
More filters
Journal ArticleDOI
ICAM-1 mediated tumor-mesothelial cell adhesion is modulated by IL-6 and TNF-α: A potential mechanism by which surgical trauma increases peritoneal metastases
TL;DR: Mesothelial ICAM-1 plays a role in the adhesion of tumor cells to the peritoneum in the development of peritoneal metastases, and is demonstrated to reduce the ability of both pancreatic and colonic cancer cell lines to adhere to the mesothelium.
Journal ArticleDOI
Targeted therapy of renal cell carcinoma: synergistic activity of cG250-TNF and IFNg.
Stefan Bauer,J.C. Oosterwijk-Wakka,Nicole Adrian,Egbert Oosterwijk,Eliane Fischer,Thomas Wüest,Frank Stenner,Angelo Perani,Leonard Cohen,Alexander Knuth,Chaitanya R. Divgi,Dirk Jäger,Andrew M. Scott,Gerd Ritter,Lloyd J. Old,Christoph Renner +15 more
TL;DR: Biodistribution studies demonstrated specific accumulation and retention of cG250‐TNF at CA‐IX‐positive RCC resulting in growth inhibition of RCC and improved progression free survival and overall survival.
Journal Article
Regulation of intercellular adhesion molecule-1 gene by tumor necrosis factor-alpha is mediated by the nuclear factor-kappaB heterodimers p65/p65 and p65/c-Rel in the absence of p50.
TL;DR: The mechanisms of transcriptional control involved in the stimulation of ICAM-1 gene expression by tumor necrosis factor-alpha (TNF-alpha) and by the nuclear factor-kappB (NF-kappaB) family of transcription factors in the Ad5-transformed human embryonal kidney cell line 293 are examined.
Journal ArticleDOI
Overcoming TNF-α and Drug Resistance of Human Renal Cell Carcinoma Cells by Treatment with Pentoxifylline in Combination with TNF-α or Drugs: The Role of TNF-α mRNA Downregulation in Tumor Cell Sensitization
Youichi Mizutani,Youichi Mizutani,Benjamin Bonavida,Benjamin Bonavida,Yoshinori Nio,Yoshinori Nio,Osamu Yoshida,Osamu Yoshida +7 more
TL;DR: This study investigates whether PTX downregulates the expression of TNF-α mRNA and/or protein in renal cell carcinoma (RCC) cells and whetherPTX enhances the sensitivity of T NF-α-resistant RCC cells to TNF -α by downregulation.
Journal ArticleDOI
Toll-Like Receptor 4 Mediates the Antitumor Host Response Induced by a 55-Kilodalton Protein Isolated from Aeginetia indica L., a Parasitic Plant
Masato Okamoto,Go Ohe,Tetsuya Oshikawa,Sachiko Furuichi,Tomoyuki Tano,Sharif Uddin Ahmed,Sachiko Akashi,Kensuke Miyake,Osamu Takeuchi,Shizuo Akira,Kunisuke Himeno,Mitsunobu Sato,Shinya Ohkubo +12 more
TL;DR: It is suggested that TLR4 mediates antitumor immunity induced by the plant-derived protein AILb-A, which was severely impaired inTLR4-deficient but not TLR2- deficient mice.