Extraintestinal Pathogenic and Antimicrobial-Resistant Escherichia coli, Including Sequence Type 131 (ST131), from Retail Chicken Breasts in the United States in 2013.
James R. Johnson,Stephen B. Porter,Brian D. Johnston,Paul Thuras,Sarah Clock,Michael Crupain,Urvashi Rangan +6 more
Reads0
Chats0
TLDR
It is found that among Escherichia coli isolates from retail chicken meat products purchased across the United States in 2013, a minority had genetic profiles suggesting an ability to cause extraintestinal infections in humans, such as urinary tract infection, implying a risk of foodborne disease.Abstract:
Chicken meat products are hypothesized to be vehicles for transmitting antimicrobial-resistant and extraintestinal pathogenic Escherichia coli (ExPEC) to consumers. To reassess this hypothesis in the current era of heightened concerns about antimicrobial use in food animals, we analyzed 175 chicken-source E. coli isolates from a 2013 Consumer Reports national survey. Isolates were screened by PCR for ExPEC-defining virulence genes. The 25 ExPEC isolates (12% of 175) and a 2:1 randomly selected set of 50 non-ExPEC isolates were assessed for their phylogenetic/clonal backgrounds and virulence genotypes for comparison with their resistance profiles and the claims on the retail packaging label ("organic," "no antibiotics," and "natural"). Compared with the findings for non-ExPEC isolates, the group of ExPEC isolates had a higher prevalence of phylogroup B2 isolates (44% versus 4%; P < 0.001) and a lower prevalence of phylogroup A isolates (4% versus 30%; P = 0.001), a higher prevalence of multiple individual virulence genes, higher virulence scores (median, 11 [range, 4 to 16] versus 8 [range, 1 to 14]; P = 0.001), and higher resistance scores (median, 4 [range, 0 to 8] versus 3 [range, 0 to 10]; P < 0.001). All five isolates of sequence type 131 (ST131) were ExPEC (P = 0.003), were as extensively resistant as the other isolates tested, and had higher virulence scores than the other isolates tested (median, 12 [range, 11 to 13] versus 8 [range, 1 to 16]; P = 0.005). Organic labeling predicted lower resistance scores (median, 2 [range, 0 to 3] versus 4 [range, 0 to 10]; P = 0.008) but no difference in ExPEC status or virulence scores. These findings document a persisting reservoir of extensively antimicrobial-resistant ExPEC isolates, including isolates from ST131, in retail chicken products in the United States, suggesting a potential public health threat.IMPORTANCE We found that among Escherichia coli isolates from retail chicken meat products purchased across the United States in 2013 (many of these isolates being extensively antibiotic resistant), a minority had genetic profiles suggesting an ability to cause extraintestinal infections in humans, such as urinary tract infection, implying a risk of foodborne disease. Although isolates from products labeled "organic" were less extensively antibiotic resistant than other isolates, they did not appear to be less virulent. These findings suggest that retail chicken products in the United States, even if they are labeled "organic," pose a potential health threat to consumers because they are contaminated with extensively antibiotic-resistant and, presumably, virulent E. coli isolates.read more
Citations
More filters
Journal ArticleDOI
Extended-spectrum β-lactamase-producing Escherichia coli in human-derived and foodchain-derived samples from England, Wales, and Scotland: an epidemiological surveillance and typing study
Michaela Day,Katie L. Hopkins,David W. Wareham,Mark Toleman,Nicola Elviss,Luke P. Randall,Christopher Teale,Paul Cleary,Camilla Wiuff,Michel Doumith,Matthew J. Ellington,Neil Woodford,David M. Livermore,David M. Livermore +13 more
TL;DR: Most human bacteraemias with ESBL-E coli in the UK involve internationally prevalent human-associated STs, particularly ST131; non-human reservoirs made little contribution to invasive human disease.
Journal ArticleDOI
Avian Pathogenic Escherichia coli (APEC): An Overview of Virulence and Pathogenesis Factors, Zoonotic Potential, and Control Strategies.
TL;DR: In this paper, the authors discuss the virulence and pathogenesis factors of APEC, review the zoonotic potential, provide the current status of antibiotic resistance and progress in vaccine development, and summarize the alternative control measures being investigated.
Journal ArticleDOI
Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections.
Shelley Gibson,Sabrina I. Green,Carmen Gu Liu,Keiko C. Salazar,Justin R. Clark,Austen Terwilliger,Heidi B. Kaplan,Anthony W. Maresso,Barbara W. Trautner,Barbara W. Trautner,Robert F. Ramig +10 more
TL;DR: The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and virulence on a number of contemporary clinical isolates are presented.
Journal ArticleDOI
Accessory Traits and Phylogenetic Background Predict Escherichia coli Extraintestinal Virulence Better Than Does Ecological Source.
James R. Johnson,James R. Johnson,Brian D. Johnston,Brian D. Johnston,Stephen B. Porter,Stephen B. Porter,Paul Thuras,Maliha Aziz,Lance B. Price +8 more
TL;DR: Among human-source E. coli isolates, specific accessory traits and phylogenetic/clonal backgrounds predict experimental virulence in a murine sepsis model better than does ecological source.
Journal ArticleDOI
Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France
Alain Rincé,Charlotte Balière,Dominique Hervio-Heath,Joelle Cozien,Solen Lozach,Sylvain Parnaudeau,Françoise S. Le Guyader,Simon Le Hello,Jean-Christophe Giard,Nicolas Sauvageot,Abdellah Benachour,Sofia Strubbia,Michèle Gourmelon +12 more
TL;DR: In this article, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments.
References
More filters
Journal ArticleDOI
The Clermont Escherichia coli phylo‐typing method revisited: improvement of specificity and detection of new phylo‐groups
Olivier Clermont,Olivier Clermont,Julia K. Christenson,Erick Denamur,Erick Denamur,David M. Gordon +5 more
TL;DR: A new PCR-based method is developed that enables an E. coli isolate to be assigned to one of the eight phylo-groups and which allows isolates that are members of the other cryptic clades (II to V) of Escherichia to be identified.
Journal ArticleDOI
Medical and economic impact of extraintestinal infections due to Escherichia coli: focus on an increasingly important endemic problem
Thomas A. Russo,James R. Johnson +1 more
TL;DR: Underappreciated features include the wide variety of extraintestinal infections E. coli can cause, the high incidence and associated morbidity, mortality, and costs of these diverse clinical syndromes, and increasing antimicrobial resistance.
Journal ArticleDOI
Proposal for a New Inclusive Designation for Extraintestinal Pathogenic Isolates of Escherichia coli: ExPEC
Thomas A. Russo,James R. Johnson +1 more
Journal ArticleDOI
The Epidemic of Extended-Spectrum-β-Lactamase-Producing Escherichia coli ST131 Is Driven by a Single Highly Pathogenic Subclone, H30-Rx
Lance B. Price,Lance B. Price,James R. Johnson,Maliha Aziz,Maliha Aziz,Connie Clabots,Brian D. Johnston,Veronika Tchesnokova,Lora Nordstrom,Maria Billig,Sujay Chattopadhyay,Marc Stegger,Marc Stegger,Paal Skytt Andersen,Paal Skytt Andersen,Talima Pearson,Kim Riddell,Peggy Rogers,Delia Scholes,Barbara C. Kahl,Paul Keim,Paul Keim,Evgeni V. Sokurenko +22 more
TL;DR: P pulsed-field gel electrophoresis and whole-genome sequencing are applied to reconstruct the evolutionary history of the ST131 clone and suggest that the high prevalence of CTX-M-15 production among ST131 isolates is due primarily to the expansion of a single, highly virulent subclone, H30-Rx.