Journal ArticleDOI
Fluorescent Probe HKSOX-1 for Imaging and Detection of Endogenous Superoxide in Live Cells and In Vivo.
Jun Jacob Hu,Nai-Kei Wong,Sen Ye,Xingmiao Chen,Mingyang Lu,Angela Qian Zhao,Yuhan Guo,Alvin C.H. Ma,Anskar Y.H. Leung,Jiangang Shen,Dan Yang +10 more
Reads0
Chats0
TLDR
Three new O2(•-) fluorescent probes are synthesized which exhibit excellent selectivity and sensitivity over a broad range of pH, strong oxidants, and abundant reductants found in cells, which open up exciting opportunities for unmasking the roles of O2 (•-) in health and disease.Abstract:
Superoxide anion radical (O2(•-)) is undoubtedly the most important primary reactive oxygen species (ROS) found in cells, whose formation and fate are intertwined with diverse physiological and pathological processes. Here we report a highly sensitive and selective O2(•-) detecting strategy involving O2(•-) cleavage of an aryl trifluoromethanesulfonate group to yield a free phenol. We have synthesized three new O2(•-) fluorescent probes (HKSOX-1, HKSOX-1r for cellular retention, and HKSOX-1m for mitochondria-targeting) which exhibit excellent selectivity and sensitivity toward O2(•-) over a broad range of pH, strong oxidants, and abundant reductants found in cells. In confocal imaging, flow cytometry, and 96-well microplate assay, HKSOX-1r has been robustly applied to detect O2(•-) in multiple cellular models, such as inflammation and mitochondrial stress. Additionally, our probes can be efficiently applied to visualize O2(•-) in intact live zebrafish embryos. These probes open up exciting opportunities for unmasking the roles of O2(•-) in health and disease.read more
Citations
More filters
Journal ArticleDOI
Fluorescent chemosensors: The past, present and future
TL;DR: The history of the development in the research of fluorescent sensors, often referred to as chemosensors, and some pioneering and representative works from about 40 groups in the world that have made substantial contributions to this field are highlighted.
Journal ArticleDOI
Recent progress in the development of fluorescent, luminescent and colorimetric probes for detection of reactive oxygen and nitrogen species
Xiaoqiang Chen,Xiaoqiang Chen,Fang Wang,Fang Wang,Ji Young Hyun,Tingwen Wei,Jian Qiang,Xintong Ren,Injae Shin,Juyoung Yoon +9 more
TL;DR: In this review, fluorescent, luminescent and colorimetric ROS and RNS probes, which have been developed since 2011, are comprehensively discussed.
Journal ArticleDOI
Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications
Jacek Zielonka,Joy Joseph,Adam Sikora,Micael Hardy,Olivier Ouari,Jeannette Vasquez-Vivar,Gang Cheng,Marcos Lopez,Balaraman Kalyanaraman +8 more
TL;DR: The physicochemical basis for mitochondrial accumulation of lipophilic cations, synthetic chemistry strategies to target compounds to mitochondria, mitochondrial probes, and sensors, and examples of mitochondrial targeting of bioactive compounds are described.
Journal ArticleDOI
Development of Ion Chemosensors Based on Porphyrin Analogues
TL;DR: This Review provides valuable information for researchers of related areas and thus may inspire the development of more practical and effective approaches for designing high-performance ion chemosensors based on porphyrin analogues and other relevant compounds.
Journal ArticleDOI
Fluorescent probes for organelle-targeted bioactive species imaging
TL;DR: The design, applications, challenges and potential directions of organelle-targeted bioactive species probes are described.
References
More filters
Book
Free radicals in biology and medicine
TL;DR: 1. Oxygen is a toxic gas - an introduction to oxygen toxicity and reactive species, and the chemistry of free radicals and related 'reactive species'
Journal ArticleDOI
Biochemistry and molecular cell biology of diabetic complications
TL;DR: This integrating paradigm provides a new conceptual framework for future research and drug discovery in diabetes-specific microvascular disease and seems to reflect a single hyperglycaemia-induced process of overproduction of superoxide by the mitochondrial electron-transport chain.
Journal ArticleDOI
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Karen Bedard,Karl-Heinz Krause +1 more
TL;DR: This review summarizes the current state of knowledge of the functions of NOX enzymes in physiology and pathology.
Journal ArticleDOI
Regulation of cancer cell metabolism
TL;DR: Interest in the topic of tumour metabolism has waxed and waned over the past century, but it has become clear that many of the signalling pathways that are affected by genetic mutations and the tumour microenvironment have a profound effect on core metabolism, making this topic once again one of the most intense areas of research in cancer biology.
Journal ArticleDOI
A role for mitochondria in NLRP3 inflammasome activation
TL;DR: It is shown that mitophagy/autophagy blockade leads to the accumulation of damaged, ROS-generating mitochondria, and this in turn activates the NLRP3 inflammasome, and may explain the frequent association of mitochondrial damage with inflammatory diseases.