Functional Myc-Max heterodimer is required for activation-induced apoptosis in T cell hybridomas.
TLDR
It is shown that coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis.Abstract:Â
T cell hybridomas respond to activation signals by undergoing apoptotic cell death, and this is likely to represent comparable events related to tolerance induction in immature and mature T cells in vivo. Previous studies using antisense oligonucleotides implicated the c-Myc protein in the phenomenon of activation-induced apoptosis. This role for c-Myc in apoptosis is now confirmed in studies using a dominant negative form of its heterodimeric binding partner, Max, which we show here inhibits activation-induced apoptosis. Further, coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis. These results imply that Myc promotes activation-induced apoptosis by obligatory heterodimerization with Max, and therefore, by regulating gene transcription.read more
Citations
More filters
Journal ArticleDOI
Function of the c-Myc oncoprotein in chromatin remodeling and transcription
TL;DR: A working hypothesis for the concerted action of HAT and SWI/SNF complexes in Myc-activated transcription is presented and it is argued that this model should prompt re-thinking of the experimental strategies and criteria used to identify Myc target genes.
Journal ArticleDOI
Signaling and transcriptional control of Fas ligand gene expression
TL;DR: Important advances in understanding are reviewed in the understanding of the signaling and transcriptional mechanisms controlling FasL gene expression.
Journal ArticleDOI
Fas (CD95/Apo-1) ligand regulation in T cell homeostasis, cell-mediated cytotoxicity and immune pathology
TL;DR: In this review, various aspects of FasL regulation in cell-mediated cytotoxicity, immune homeostasis and the immunopathology of diseases are discussed.
Journal ArticleDOI
Apoptotic action of estrogen.
R. X.-D. Song,Richard J. Santen +1 more
TL;DR: This mini review will focus on the latest findings regarding estrogen apoptotic effects in several cell models, including breast cancer cells, and summarize the possible mechanisms involved in these estrogen mediated processes.
Journal ArticleDOI
DEC1 Negatively Regulates the Expression of DEC2 through Binding to the E-box in the Proximal Promoter
TL;DR: DDEC1-mediated repression on the expression of DEC2 provides an important mechanism that these transcription factors regulate the cellular function not only by modulating the Expression of their target genes but also theexpression of members within the same class.
References
More filters
Journal Article
T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity
TL;DR: This highly reproducible, quantitative assay for T cell growth factor (TCGF), based upon the tritiated-thymidine incorporation of continuous murine tumor-specific cytotoxic T cell lines (CTLL), has revealed that T lymphocytes are required for its production.
Journal ArticleDOI
Induction of apoptosis in fibroblasts by c-myc protein
Gerard I. Evan,Andrew H. Wyllie,Christopher S. Gilbert,Trevor Littlewood,Hartmut Land,Mary W. Brooks,Catherine M. Waters,Linda Z. Penn,David C. Hancock +8 more
TL;DR: It is demonstrated that deregulated c-myc expression induces apoptosis in cells growth arrested by a variety of means and at various points in the cell cycle.
Journal ArticleDOI
Features of apoptotic cells measured by flow cytometry
Zbigniew Darzynkiewicz,S. Bruno,G. Del Bino,Wojciech Gorczyca,Michel A. Hotz,P. Lassota,Frank Traganos +6 more
TL;DR: The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis, applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA topoisomerase I or II inhibitors, and in rat thymocytes by either topoisomersase inhibitors or prednisolone.
PatentDOI
Max: a helix-loop-helix zipper protein that forms a sequence-specific dna-binding complex with myc and mad
TL;DR: In this paper, the Max polypeptide when associated with the Myc or Mad polyPEptide is capable of binding to nucleotide sequences containing CACGTG.
Journal ArticleDOI
Identification of a monoclonal antibody specific for a murine T3 polypeptide
TL;DR: Results identify T3-epsilon as a cell surface protein involved in the transduction of activation signals and can both activate and inhibit T-cell function.