Functional Myc-Max heterodimer is required for activation-induced apoptosis in T cell hybridomas.
TLDR
It is shown that coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis.Abstract:
T cell hybridomas respond to activation signals by undergoing apoptotic cell death, and this is likely to represent comparable events related to tolerance induction in immature and mature T cells in vivo. Previous studies using antisense oligonucleotides implicated the c-Myc protein in the phenomenon of activation-induced apoptosis. This role for c-Myc in apoptosis is now confirmed in studies using a dominant negative form of its heterodimeric binding partner, Max, which we show here inhibits activation-induced apoptosis. Further, coexpression of a reciprocally mutant Myc protein capable of forming functional heterodimers with the mutant Max can compensate for the dominant negative activity and restore activation-induced apoptosis. These results imply that Myc promotes activation-induced apoptosis by obligatory heterodimerization with Max, and therefore, by regulating gene transcription.read more
Citations
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Expression of Fas ligand in activated T cells is regulated by c-Myc.
Thomas Brunner,Shailaja Kasibhatla,Michael J. Pinkoski,Corina Frutschi,Nam Jin Yoo,Fernando Echeverri,Artin Mahboubi,Douglas R. Green +7 more
TL;DR: It is demonstrated that T cell activation-induced expression of Fas ligand (FasL, CD95-L, APO-1-L), which can induce apoptotic cell death in many different cell types, is regulated by c-Myc.
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Jak3, STAT3, and STAT5 inhibit expression of miR-22, a novel tumor suppressor microRNA, in cutaneous T-Cell lymphoma
Nina A. Sibbesen,Katharina L. Kopp,Ivan V. Litvinov,Lars Jønson,Andreas Willerslev-Olsen,Simon Fredholm,David L. Petersen,Claudia Nastasi,Thorbjørn Krejsgaard,Lise M. Lindahl,Robert Gniadecki,Nigel P. Mongan,Denis Sasseville,Mariusz A. Wasik,Lars Iversen,Charlotte M. Bonefeld,Carsten Geisler,Anders Woetmann,Niels Ødum +18 more
TL;DR: The first evidence that de-regulated Jak3/STAT3/ STAT5 signalling in CTCL cells represses the expression of the gene encoding miR-22, a novel tumor suppressor miRNA is provided.
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Cell death in the regulation of immune responses.
TL;DR: A myriad of proteins that can initiate or protect cells from cell death have recently been identified.
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Cyclin D3 sensitizes tumor cells to tumor necrosis factor-induced, c-Myc-dependent apoptosis.
TL;DR: Results show that cyclin D3 sensitizes tumor cells to TNF-induced apoptosis and indicate that the expression of c-Myc and expression of cycl in D3 in HeLa and in HT-1080 fibrosarcoma cells are closely linked.
References
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Journal Article
T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity
TL;DR: This highly reproducible, quantitative assay for T cell growth factor (TCGF), based upon the tritiated-thymidine incorporation of continuous murine tumor-specific cytotoxic T cell lines (CTLL), has revealed that T lymphocytes are required for its production.
Journal ArticleDOI
Induction of apoptosis in fibroblasts by c-myc protein
Gerard I. Evan,Andrew H. Wyllie,Christopher S. Gilbert,Trevor Littlewood,Hartmut Land,Mary W. Brooks,Catherine M. Waters,Linda Z. Penn,David C. Hancock +8 more
TL;DR: It is demonstrated that deregulated c-myc expression induces apoptosis in cells growth arrested by a variety of means and at various points in the cell cycle.
Journal ArticleDOI
Features of apoptotic cells measured by flow cytometry
Zbigniew Darzynkiewicz,S. Bruno,G. Del Bino,Wojciech Gorczyca,Michel A. Hotz,P. Lassota,Frank Traganos +6 more
TL;DR: The present review describes several methods to characterize and differentiate between two different mechanisms of cell death, apoptosis and necrosis, applied to studies of apoptosis triggered in the human leukemic HL-60 cell line by DNA topoisomerase I or II inhibitors, and in rat thymocytes by either topoisomersase inhibitors or prednisolone.
PatentDOI
Max: a helix-loop-helix zipper protein that forms a sequence-specific dna-binding complex with myc and mad
TL;DR: In this paper, the Max polypeptide when associated with the Myc or Mad polyPEptide is capable of binding to nucleotide sequences containing CACGTG.
Journal ArticleDOI
Identification of a monoclonal antibody specific for a murine T3 polypeptide
TL;DR: Results identify T3-epsilon as a cell surface protein involved in the transduction of activation signals and can both activate and inhibit T-cell function.