Journal ArticleDOI
Gene manipulation through the use of small interfering RNA (siRNA): from in vitro to in vivo applications.
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TLDR
The rationale and different strategies of using siRNA/shRNA for accomplishing the silencing of targeted genes in a spatial and /or temporally regulated manner are discussed and the steps involved in extending these approaches to in vivo applications are summarised.About:
This article is published in Advanced Drug Delivery Reviews.The article was published on 2007-03-30. It has received 89 citations till now. The article focuses on the topics: Small interfering RNA & RNA interference.read more
Citations
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Journal ArticleDOI
Chitosan-based formulations for delivery of DNA and siRNA.
Shirui Mao,Wei Sun,Thomas Kissel +2 more
TL;DR: Chitosan structure modification or additive incorporation is an effective way to improve the stability of the polyplex in biological fluids, enhance targeted cell delivery and facilitate endo-lysosomal release of the complex.
Journal ArticleDOI
Lipid-based systemic delivery of siRNA.
TL;DR: This review discusses the challenges in systemic siRNA delivery by dividing them into two categories-kinetic and physical barriers and different strategies to overcome these barriers, especially focusing on the step of endosome escape.
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Nanotoxicity: a key obstacle to clinical translation of siRNA-based nanomedicine
TL;DR: The nanotoxicity of siRNA carriers is reviewed to help clear the way for siRNAs to fulfill their promise as a versatile class of therapeutic agents.
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Biodegradable amphiphilic poly(ethylene oxide)-block-polyesters with grafted polyamines as supramolecular nanocarriers for efficient siRNA delivery
TL;DR: The results of this study demonstrated the promise of novel amphiphilic PEO-b-P(CL-g-polyamine) block copolymers for efficient siRNA delivery and showed effective endosomal escape after cellular uptake.
Journal ArticleDOI
Lipid-Based Nanocarriers for RNA Delivery
TL;DR: Focus of the discussion is on the various challenges researchers face when developing lipid-based RNA nanocarriers, such as the toxicity of cationic lipids and issues related to PEGylated lipids, as well as the strategies employed in tackling these challenges.
References
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Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
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In vitro selection of RNA molecules that bind specific ligands.
TL;DR: Subpopulations of RNA molecules that bind specifically to a variety of organic dyes have been isolated from a population of random sequence RNA molecules.
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Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
TL;DR: It is shown that mammalian TLR3 recognizes dsRNA, and that activation of the receptor induces the activation of NF-κB and the production of type I interferons (IFNs).
Journal ArticleDOI
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Manfred Gossen,Hermann Bujard +1 more
TL;DR: Control elements of the tetracycline-resistance operon encoded in Tn10 of Escherichia coli have been utilized to establish a highly efficient regulatory system in mammalian cells that is suitable for creation of "on/off" situations for such genes in a reversible way.
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