Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.
Matthew S. Walters,Kazunori Gomi,Beth Ashbridge,Malcolm A.S. Moore,Vanessa Arbelaez,Jonna Heldrich,Bi-Sen Ding,Shahin Rafii,Michelle R. Staudt,Ronald G. Crystal +9 more
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TLDR
Development of immortalized human airway BC that retain multipotent differentiation capacity over long-term culture should be useful in understanding the biology of BC, the response of BC to environmental stress, and as a target for assessment of pharmacologic agents.Abstract:
Background
As the multipotent progenitor population of the airway epithelium, human airway basal cells (BC) replenish the specialized differentiated cell populations of the mucociliated airway epithelium during physiological turnover and repair. Cultured primary BC divide a limited number of times before entering a state of replicative senescence, preventing the establishment of long-term replicating cultures of airway BC that maintain their original phenotype.read more
Citations
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Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells.
Hongmei Mou,Vladimir Vinarsky,Purushothama Rao Tata,Karissa Brazauskas,Soon H. Choi,Adrianne K. Crooke,Bing Zhang,George M. Solomon,Brett Turner,Hermann Bihler,Jan Harrington,Allen Lapey,Colleen L. Channick,Colleen Keyes,Adam Freund,Steven E. Artandi,Martin Mense,Steven M. Rowe,John F. Engelhardt,Ya-Chieh Hsu,Jayaraj Rajagopal +20 more
TL;DR: This work shows that dual inhibition of SMAD signaling pathways enables robust expansion of primary epithelial basal cell populations in airway epithelia, and finds that TGFβ/BMP/SMAD pathway signaling is strongly activated in luminal and suprabasal cells of several epithelium, but suppressed in p63+ basal cells.
Journal ArticleDOI
Type I Interferon Susceptibility Distinguishes SARS-CoV-2 from SARS-CoV.
Kumari G. Lokugamage,Adam Hage,Maren de Vries,Ana M. Valero-Jimenez,Craig Schindewolf,Meike Dittmann,Ricardo Rajsbaum,Vineet D. Menachery +7 more
TL;DR: Evaluating type I interferon (IFN-I) sensitivity of SARS-CoV-2 relative to the original SARS -CoV indicates that while Sars-Cov-2 maintains similar viral replication to SARsCoV, the novel CoV is much more sensitive to IFn-I.
Posted ContentDOI
SARS-CoV-2 sensitive to type I interferon pretreatment.
Kumari G. Lokugamage,Adam Hage,Maren Devries,Ana M Vallero-Jimenez,Craig Schindewolf,Meike Dittmann,Ricardo Rajsbaum,Vineet D. Menachery +7 more
TL;DR: Evaluating type-I Interferon (IFN-I) sensitivity of SARS-CoV-2 relative to the original SARS -CoV indicates the novel CoV is much more sensitive to IFn-I pretreatment, while the absence of open reading frame (ORF) 3b and significant changes to ORF6 suggest the two key IFN antagonists may not maintain equivalent function in SARS.
Journal ArticleDOI
Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium.
Haijun Zhang,Mahboubeh Rostami,Philip L. Leopold,Jason G. Mezey,Sarah L. O’Beirne,Yael Strulovici-Barel,Ronald G. Crystal +6 more
TL;DR: ACE2, the gene encoding the receptor for SARS-CoV-2, is expressed in the human airway epithelium, with variations in expression relevant to the biology of initial steps in Sars-Co V-2 infection.
Posted ContentDOI
Type I interferon susceptibility distinguishes SARS-CoV-2 from SARS-CoV
Kumari G. Lokugamage,Adam Hage,Maren de Vries,Ana M. Valero-Jimenez,Craig Schindewolf,Meike Dittmann,Ricardo Rajsbaum,Vineet D. Menachery +7 more
TL;DR: SARS-CoV-2 has similar replication kinetics to SARS- CoV, but demonstrates significant sensitivity to type I interferon treatment, which could help inform disease progression, treatment options, and animal model development.
References
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Jay P. Morgenstern,Hartmut Land +1 more
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Jason R. Rock,Mark W. Onaitis,Emma L. Rawlins,Yun Lu,Cheryl P. Clark,Yan Xue,Scott H. Randell,Brigid L.M. Hogan +7 more
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Transformation of Human Bronchial Epithelial Cells by Infection with SV40 or Adenovirus-12 SV40 Hybrid Virus, or Transfection via Strontium Phosphate Coprecipitation with a Plasmid Containing SV40 Early Region Genes
Roger R. Reddel,Yang Ke,Brenda I. Gerwin,Mary G. McMenamin,John F. Lechner,Robert T. Su,Douglas E. Brash,Joo-Bae Park,Johng S. Rhim,Curtis C. Harris +9 more
TL;DR: Normal human bronchial epithelial cells were infected with SV40 virus or an adenovirus 12-SV40 hybrid virus, or transfected via strontium phosphate coprecipitation with plasmids containing the SV40 early region genes, and Colonies of morphologically altered cells were isolated and cultured.
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The airway epithelium in asthma
Bart N. Lambrecht,Hamida Hammad +1 more
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