Generation of Functional Murine Cardiac Myocytes From Induced Pluripotent Stem Cells
Christina Mauritz,Kristin Schwanke,Michael Reppel,Stefan Neef,K. Katsirntaki,Lars S. Maier,Filomain Nguemo,Sandra Menke,Moritz Haustein,Juergen Hescheler,Gerd Hasenfuss,Ulrich Martin +11 more
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TLDR
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line, and found that iPS cells differentiate into functional cardiomyocytes.Abstract:
Background— The recent breakthrough in the generation of induced pluripotent stem (iPS) cells, which are almost indistinguishable from embryonic stem (ES) cells, facilitates the generation of murine disease– and human patient–specific stem cell lines. The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line. Methods and Results— With the use of a standard embryoid body–based differentiation protocol for ES cells, iPS cells as well as ES cells were differentiated for 24 days. Although the analyzed iPS cell clone showed a delayed and less efficient formation of beating embryoid bodies compared with the ES cell line, the differentiation resulted in an average of 55% of spontaneously contracting iPS cell embryoid bodies. Analyses on molecular, structural, and functional levels demonstrated that iPS cell–derived cardiomyocytes show typical features of ES cell–derived cardiomyocytes. Reverse transcription p...read more
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Long QT Syndrome: Genetics and Future Perspective
Eimear Wallace,Linda Howard,Min Liu,Timothy O'Brien,Deirdre Ward,Sanbing Shen,Terence Prendiville +6 more
TL;DR: It is now possible to model the various LQTS phenotypes through the generation of patient-specific induced pluripotent stem cell-derived cardiomyocytes and CRISPR/Cas9 is a genome editing technology that offers great potential in elucidating gene function and a potential therapeutic strategy for monogenic disease.
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c-MYC-Independent Nuclear Reprogramming Favors Cardiogenic Potential of Induced Pluripotent Stem Cells
TL;DR: Nuclear reprogramming independent of c-MYC enhances production of pluripotent stem cells with innate cardiogenic potential and achieves early and robust cardiogenesis during in vitro differentiation with consistent beating activity, sarcomere maturation, and rhythmical intracellular calcium dynamics.
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Engineering cell platforms for myocardial regeneration
Udi Sarig,Marcelle Machluf +1 more
TL;DR: The combined cell-biomaterial scaffold therapy is superior to cell therapy alone and the development of complementary dynamic 3D cultivation platforms will probably lead to improved outcomes and enable fast screening of various therapeutic approaches.
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In vitro Model for Assessing Arrhythmogenic Properties of Drugs Based on High-resolution Impedance Measurements
TL;DR: In this article, the authors combined two emerging technologies, induced pluripotent stem (iPS) cell-derived CMs and impedance-based real-time (xCELLigence RTCA Cardio Instrument) monitoring of CM electrical activity, to assess the effect of drugs known affect cardiac activity such as isoproterenol, carbachol, terfenadine, sotalol and doxorubicin.
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The potential and challenges of using stem cells for cardiovascular repair and regeneration.
TL;DR: Advantages and disadvantages of currently-used stem cells for cardiovascular repair and regeneration are discussed and the limitations and uniqueness of some types of stem cells will be discussed.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
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Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.