Generation of Functional Murine Cardiac Myocytes From Induced Pluripotent Stem Cells
Christina Mauritz,Kristin Schwanke,Michael Reppel,Stefan Neef,K. Katsirntaki,Lars S. Maier,Filomain Nguemo,Sandra Menke,Moritz Haustein,Juergen Hescheler,Gerd Hasenfuss,Ulrich Martin +11 more
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TLDR
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line, and found that iPS cells differentiate into functional cardiomyocytes.Abstract:
Background— The recent breakthrough in the generation of induced pluripotent stem (iPS) cells, which are almost indistinguishable from embryonic stem (ES) cells, facilitates the generation of murine disease– and human patient–specific stem cell lines. The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line. Methods and Results— With the use of a standard embryoid body–based differentiation protocol for ES cells, iPS cells as well as ES cells were differentiated for 24 days. Although the analyzed iPS cell clone showed a delayed and less efficient formation of beating embryoid bodies compared with the ES cell line, the differentiation resulted in an average of 55% of spontaneously contracting iPS cell embryoid bodies. Analyses on molecular, structural, and functional levels demonstrated that iPS cell–derived cardiomyocytes show typical features of ES cell–derived cardiomyocytes. Reverse transcription p...read more
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Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling.
TL;DR: Recent findings regarding the use of iPS-derived cells for modeling several genetic cardiac disorders have indicated that these pluripotent cells represent an ideal tool for drug testing and might contribute to the development of future personalized regenerative cell therapies.
New Methods in Cardiovascular Biology Reporter-Based Isolation of Induced Pluripotent Stem Cell- and Embryonic Stem Cell-Derived Cardiac Progenitors Reveals Limited Gene Expression Variance
Linda W. van Laake,Li Qian,Paul Cheng,Yu Huang,Edward C. Hsiao,Bruce R. Conklin,Deepak Srivastava +6 more
TL;DR: In this paper, the authors investigated how much variability exists between differentiated lineages from independent iPS cell lines and how similar iPS-derived cardiomyocytes are to ES-CMs.
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Mesenchymal stem cell: present challenges and prospective cellular cardiomyoplasty approaches for myocardial regeneration.
TL;DR: In this review, an in-depth discussion on the complexities associated with stem and progenitor cell therapies, the potential of preclinical approaches involving MSCs for myocardial repair, and an account of the past milestones and ongoing MSC-based trials in humans are presented.
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Human Pluripotent Stem Cell-Derived Cardiomyocytes: Response to TTX and Lidocain Reveals Strong Cell to Cell Variability
Xiaowu Sheng,Xiaowu Sheng,Michael Reppel,Filomain Nguemo,Farooq I. Mohammad,Farooq I. Mohammad,Alexey Kuzmenkin,Jürgen Hescheler,Kurt Pfannkuche +8 more
TL;DR: Electrophysiological properties of hESC-CMs (HES2) and hiPSC- CMs, derived from reprogrammed adult foreskin fibroblasts, are analyzed and substantial differences in action potentials (APs) and sodium currents at late stage of in vitro differentiation with higher sodium currents in hiPSCs are found.
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Advances in understanding the mechanism of zebrafish heart regeneration
TL;DR: This review surveys recent advances in the field and discusses current understanding of the endogenous mechanisms of cardiac regeneration in zebrafish, which is arguably the best characterized with respect to cardiac regenerative responses.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.