Generation of Functional Murine Cardiac Myocytes From Induced Pluripotent Stem Cells
Christina Mauritz,Kristin Schwanke,Michael Reppel,Stefan Neef,K. Katsirntaki,Lars S. Maier,Filomain Nguemo,Sandra Menke,Moritz Haustein,Juergen Hescheler,Gerd Hasenfuss,Ulrich Martin +11 more
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TLDR
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line, and found that iPS cells differentiate into functional cardiomyocytes.Abstract:
Background— The recent breakthrough in the generation of induced pluripotent stem (iPS) cells, which are almost indistinguishable from embryonic stem (ES) cells, facilitates the generation of murine disease– and human patient–specific stem cell lines. The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line. Methods and Results— With the use of a standard embryoid body–based differentiation protocol for ES cells, iPS cells as well as ES cells were differentiated for 24 days. Although the analyzed iPS cell clone showed a delayed and less efficient formation of beating embryoid bodies compared with the ES cell line, the differentiation resulted in an average of 55% of spontaneously contracting iPS cell embryoid bodies. Analyses on molecular, structural, and functional levels demonstrated that iPS cell–derived cardiomyocytes show typical features of ES cell–derived cardiomyocytes. Reverse transcription p...read more
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Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro.
TL;DR: Some intracellular factors in iPS cells inhibit the terminal differentiation of oligodendrocytes from the OPC stage, suggesting that this technique as a novel therapeutic tool in regenerative medicine is in doubt.
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Direct reprogramming of mouse fibroblasts to cardiomyocyte-like cells using Yamanaka factors on engineered poly(ethylene glycol) (PEG) hydrogels.
Amanda W. Smith,Jake D. Hoyne,Peter K. Nguyen,Dylan A. McCreedy,Haytham Aly,Igor R. Efimov,Stacey Rentschler,Donald L. Elbert +7 more
TL;DR: PEG hydrogels improved proliferation and reprogramming efficiency and designed poly(ethylene glycol) culture substrates to promote MEF adhesion via laminin and RGD-binding integrins to illustrate that cellular reprograming may be enhanced using custom-engineered materials.
PatentDOI
Induced pluripotent stem cells
Yasuhiro Ikeda,Andre Terzic,Timothy J. Nelson,Amber A. Mael,Almudena J. Martinez Fernandez,Satsuki Yamada +5 more
TL;DR: This work has shown that the reprogramming of somatic cells via transduction with viral vectors expressing the Oct4, Sox2, c-Myc, Klf4, Nanog, and Lin28 genes, which are essential for the establishment and maintenance of the pluripotent state, are highly promising for research and medicine.
Journal ArticleDOI
The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
Carlos O Heras-Bautista,Alisa Katsen-Globa,Nils E. Schloerer,Sabine Dieluweit,Osama M. Abd El Aziz,Osama M. Abd El Aziz,Gabriel Peinkofer,Wael Attia,Wael Attia,Markus Khalil,Konrad Brockmeier,Jürgen Hescheler,Kurt Pfannkuche +12 more
TL;DR: An easy method to generate polyacrylamide gels with a physiological Young's modulus of 55 kPa and defined surface ligand is presented, facilitating the culture of murine and human iPS-CMs, removing excess mechanical stresses and reducing the risk of tissue culture artifacts exerted by stiff substrates.
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Endothelial Cells Derived From Nuclear Reprogramming
TL;DR: This review focuses on the generation of ECs from stem cells; their characterization by genetic, histological, and functional studies; and their translational applications.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.