Generation of Functional Murine Cardiac Myocytes From Induced Pluripotent Stem Cells
Christina Mauritz,Kristin Schwanke,Michael Reppel,Stefan Neef,K. Katsirntaki,Lars S. Maier,Filomain Nguemo,Sandra Menke,Moritz Haustein,Juergen Hescheler,Gerd Hasenfuss,Ulrich Martin +11 more
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TLDR
The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line, and found that iPS cells differentiate into functional cardiomyocytes.Abstract:
Background— The recent breakthrough in the generation of induced pluripotent stem (iPS) cells, which are almost indistinguishable from embryonic stem (ES) cells, facilitates the generation of murine disease– and human patient–specific stem cell lines. The aim of this study was to characterize the cardiac differentiation potential of a murine iPS cell clone in comparison to a well-established murine ES cell line. Methods and Results— With the use of a standard embryoid body–based differentiation protocol for ES cells, iPS cells as well as ES cells were differentiated for 24 days. Although the analyzed iPS cell clone showed a delayed and less efficient formation of beating embryoid bodies compared with the ES cell line, the differentiation resulted in an average of 55% of spontaneously contracting iPS cell embryoid bodies. Analyses on molecular, structural, and functional levels demonstrated that iPS cell–derived cardiomyocytes show typical features of ES cell–derived cardiomyocytes. Reverse transcription p...read more
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Bone-Derived Stem Cells Repair the Heart After Myocardial Infarction Through Transdifferentiation and Paracrine Signaling Mechanisms
Jason M. Duran,Catherine A. Makarewich,Thomas E. Sharp,Timothy Starosta,Fang Zhu,Nicholas E. Hoffman,Yumi Chiba,Muniswamy Madesh,Remus M. Berretta,Hajime Kubo,Steven R. Houser +10 more
TL;DR: CBSC therapy improved survival, cardiac function, regional strain, attenuated remodeling, and decreased infarct size relative to cardiac-derived stem cells– or saline-treated myocardial infarction controls through the following 2 mechanisms: secretion of proangiogenic factors that stimulate endogenous neovascularization, and differentiation into functional adult myocytes and vascular cells.
Journal ArticleDOI
Transplantation and Tracking of Human-Induced Pluripotent Stem Cells in a Pig Model of Myocardial Infarction Assessment of Cell Survival, Engraftment, and Distribution by Hybrid Single Photon Emission Computed Tomography/Computed Tomography of Sodium Iodide Symporter Transgene Expression
Christian Templin,Robert Zweigerdt,Kristin Schwanke,Ruth Olmer,Jelena-Rima Ghadri,Maximilian Y. Emmert,Ennio Müller,Silke M. Küest,Susan Cohrs,Roger Schibli,Peter W Kronen,Monika Hilbe,Andreas Reinisch,Dirk Strunk,Axel Haverich,Simon P. Hoerstrup,Thomas F. Lüscher,Philipp A. Kaufmann,Ulf Landmesser,Ulrich Martin +19 more
TL;DR: The feasibility of repeated in vivo imaging of viability and tissue distribution of cellular grafts in large animals is described for the first time and vascular differentiation and long-term engraftment of hiPSCs in a large-animal model of myocardial infarction is demonstrated.
Journal ArticleDOI
Mesp1: A Key Regulator of Cardiovascular Lineage Commitment
Antoine Bondue,Cédric Blanpain +1 more
TL;DR: The recent findings suggesting that Mesp1 acts as a key regulator of cardiovascular progenitors in vertebrates will be very useful to boost the production of cardiovascular cells for cellular therapy, drug and toxicity screening and to understand the root of cardiovascular development.
Journal ArticleDOI
Induced pluripotent stem cell (iPSC)-derived Flk-1 progenitor cells engraft, differentiate, and improve heart function in a mouse model of acute myocardial infarction
Christina Mauritz,Andreas Martens,Sebastian V. Rojas,Tilman Schnick,Christian Rathert,Natalie Schecker,Sandra Menke,Silke Glage,Robert Zweigerdt,Axel Haverich,Ulrich Martin,Ingo Kutschka +11 more
TL;DR: A first proof of concept study evaluates the potential of a murine iPSC-derived cardiovascular progenitor population, which expresses the surface marker foetal liver kinase-1 (Flk-1), to restore myocardial tissue and improve cardiac function after acuteMyocardial infarction (MI) in mice and paves the way for an autologous i PSC-based therapy of MI.
Journal ArticleDOI
Chitosan/silk fibroin modified nanofibrous patches with mesenchymal stem cells prevent heart remodeling post-myocardial infarction in rats.
Jiangwei Chen,Yingfei Zhan,Yabin Wang,Dong Han,Bo Tao,Zhenli Luo,Sai Ma,Qun Wang,Xiang Li,Li Fan,Congye Li,Hongbing Deng,Feng Cao,Feng Cao +13 more
TL;DR: It is suggested that CS/SF-modified nanofibrous patches promote the functional survival of engrafted AD-MSCs and restrain ventricular remodeling post-MI through attenuating myocardial fibrosis.
References
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TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Bone marrow cells regenerate infarcted myocardium
Donald Orlic,Jan Kajstura,Stefano Chimenti,Igor Jakoniuk,Stacie M. Anderson,Baosheng Li,James Pickel,Ronald D.G. McKay,Bernardo Nadal-Ginard,David M. Bodine,Annarosa Leri,Piero Anversa +11 more
TL;DR: It is indicated that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.