Generation of oligodendroglial cells by direct lineage conversion
Nan Yang,J. Bradley Zuchero,Henrik Ahlenius,Samuele Marro,Yi Han Ng,Thomas Vierbuchen,John S. Hawkins,Richard Geissler,Ben A. Barres,Marius Wernig +9 more
TLDR
The generation of induced OPCs (iOPCs) by direct lineage conversion was reported, which gave rise to mature oligodendrocytes that could ensheath multiple host axons when co-cultured with primary dorsal root ganglion cells and formed myelin after transplantation into shiverer mice.Abstract:
Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available Here we report the generation of induced OPCs (iOPCs) by direct lineage conversion Forced expression of the three transcription factors Sox10, Olig2 and Zfp536 was sufficient to reprogram mouse and rat fibroblasts into iOPCs with morphologies and gene expression signatures resembling primary OPCs More importantly, iOPCs gave rise to mature oligodendrocytes that could ensheath multiple host axons when co-cultured with primary dorsal root ganglion cells and formed myelin after transplantation into shiverer mice We propose direct lineage reprogramming as a viable alternative approach for the generation of OPCs for use in disease modeling and regenerative medicineread more
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Journal ArticleDOI
OCT4-induced oligodendrocyte progenitor cells promote remyelination and ameliorate disease
Wonjin Yun,Kyung Ah Choi,Insik Hwang,Jie Zheng,Minji Park,Wonjun Hong,A-Young Jang,Jeong Hee Kim,Wonji Choi,Dae-Sung Kim,In Yong Kim,Yong Jun Kim,Yang Liu,Byung Sun Yoon,Gyu Ju Park,Gwonhwa Song,Sunghoi Hong,Seungkwon You +17 more
TL;DR: In this paper , a combination of OCT4 and a small molecule cocktail was used to generate O2B5+ (an early marker for OPCs) iOPCs within 2 weeks retaining the ability to differentiate into MBP-positive mature oligodendrocytes.
Book ChapterDOI
Stem Cell Therapies for Cervical Spinal Cord Injury
TL;DR: Three different cell types currently used in cellular transplantation strategies to treat cervical SCIs are discussed: mesenchymal stem cells, embryonic stem cells (ESCs), and induced pluripotent stem cell (iPSCs) to provide a detailed understanding of both their capabilities and also their potential drawbacks in experimental and clinical settings.
Journal ArticleDOI
Direct Conversion to Achieve Glial Cell Fates: Oligodendrocytes and Schwann Cells
TL;DR: Current achievements in obtaining glia, particularly oligodendrocytes and Schwann cells are summarized, showing that as an alternative method, it is now possible to obtain a variety of mature cell populations that could not be obtained using conventional differentiation methods.
Journal ArticleDOI
A general computational approach to predicting synergistic transcriptional cores that determine cell subpopulation identities.
Satoshi Okawa,Antonio del Sol +1 more
TL;DR: A new computational method that requires only single-cell RNA-seq data as input, and does not rely on any prior knowledge of candidate genes or gene regulatory networks, and can be directly applied to any cellular systems, including those containing novel subpopulations.
Journal ArticleDOI
Recent advances in regenerative medicine approaches for spinal cord injuries
TL;DR: Cell and drug delivery strategies can induce changes in the local microenvironment at and around the lesion site to promote transplanted cell survival, integration, and/or endogenous repair and provide a platform for sustained delivery of otherwise unstable drugs.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
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