Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TLDR
This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.Abstract:
If it were possible to reprogram differentiated human somatic cells into a pluripotent state, patient-specific and disease-specific stem cells could be developed. Previous work generated induced pluripotent stem (iPS) cells capable of germline transmission from murine somatic cells by transdread more
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
Masato Nakagawa,Michiyo Koyanagi,Koji Tanabe,Kazutoshi Takahashi,Tomoko Ichisaka,Takashi Aoi,Keisuke Okita,Yuji Mochiduki,Nanako Takizawa,Shinya Yamanaka +9 more
TL;DR: A modified protocol for the generation of iPS cells that does not require the Myc retrovirus is described and, with this protocol, significantly fewer non-iPS background cells are obtained, and theiPS cells generated were consistently of high quality.
Journal ArticleDOI
Disease-Specific Induced Pluripotent Stem Cells
In-Hyun Park,Natasha Arora,Hongguang Huo,Nimet Maherali,Tim Ahfeldt,Tim Ahfeldt,Akiko Shimamura,M. William Lensch,M. William Lensch,Chad A. Cowan,Konrad Hochedlinger,George Q. Daley +11 more
TL;DR: The generation of induced pluripotent stem cells from patients with a variety of genetic diseases with either Mendelian or complex inheritance are described, offering an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development.
Journal ArticleDOI
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Prashant Mali,John Aach,P. Benjamin Stranges,Kevin M. Esvelt,Mark Moosburner,Sriram Kosuri,Luhan Yang,George M. Church,George M. Church +8 more
TL;DR: This system is engineer to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).
Journal ArticleDOI
Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development.
Charles E. Murry,Gordon Keller +1 more
TL;DR: The potential to generate virtually any differentiated cell type from embryonic stem cells (ESCs) offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine, but it is essential to be able to control ESC differentiation and to direct the development of these cells along specific pathways.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells.
Yuin-Han Loh,Qiang Wu,Joon Lin Chew,Joon Lin Chew,Vinsensius B. Vega,Weiwei Zhang,Weiwei Zhang,Xi Chen,Xi Chen,Guillaume Bourque,Joshy George,Bernard Leong,Jun Liu,Kee Yew Wong,Ken W. Sung,Charlie W.H. Lee,Xiao Dong Zhao,Kuo Ping Chiu,Leonard Lipovich,Vladimir A. Kuznetsov,Paul Robson,Paul Robson,Lawrence W. Stanton,Chia-Lin Wei,Yijun Ruan,Bing Lim,Bing Lim,Huck-Hui Ng,Huck-Hui Ng +28 more
TL;DR: By integrating RNA interference–mediated depletion of Oct4 and Nanog with microarray expression profiling, it is demonstrated that these factors can activate or suppress transcription, and it is shown that common core downstream targets are important to keep ES cells from differentiating.
Journal ArticleDOI
New cell lines from mouse epiblast share defining features with human embryonic stem cells
Paul J. Tesar,Josh G. Chenoweth,F. A. Brook,Timothy J. Davies,Edward P. Evans,David L. Mack,Richard L. Gardner,Ronald D.G. McKay +7 more
TL;DR: It is shown that cell lines can be derived from the epiblast, a tissue of the post-implantation embryo that generates the embryo proper, and interrogated to understand how pluripotent cells generate distinct fates during early development.
Journal ArticleDOI
Clonally Derived Human Embryonic Stem Cell Lines Maintain Pluripotency and Proliferative Potential for Prolonged Periods of Culture
Michal Amit,Melissa K. Carpenter,Margaret S. Inokuma,Choy-Pik Chiu,Charles P. Harris,Michelle A. Waknitz,Joseph Itskovitz-Eldor,James A. Thomson +7 more
TL;DR: The clonal derivation of two human ES cell lines, H9.1 and H.2, demonstrates the pluripotency of single human ES cells, the maintenance of pluripOTency during an extended period of culture, and the long-term self-renewing properties of cultured human ES Cells.
Neurotechnique Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell-Derived Inducing Activity
Hiroshi Kawasaki,Kenji Mizuseki,Satomi Nishikawa,Satoshi Kaneko,Yoshihisa Kuwana,Shigetada Nakanishi,Shin-Ichi Nishikawa,Yoshiki Sasai,Kyowa Hakko Kogyo +8 more
TL;DR: In this article, an efficient system for in vitro differentiation of mouse ES cells without use of either retinoic acid or embryoid vitro neural differentiation in a serum- bodies was introduced. But this method requires neither EBs nor RA treat- phogen in Xenopus.