Generation of oligodendroglial cells by direct lineage conversion
Nan Yang,J. Bradley Zuchero,Henrik Ahlenius,Samuele Marro,Yi Han Ng,Thomas Vierbuchen,John S. Hawkins,Richard Geissler,Ben A. Barres,Marius Wernig +9 more
TLDR
The generation of induced OPCs (iOPCs) by direct lineage conversion was reported, which gave rise to mature oligodendrocytes that could ensheath multiple host axons when co-cultured with primary dorsal root ganglion cells and formed myelin after transplantation into shiverer mice.Abstract:
Transplantation of oligodendrocyte precursor cells (OPCs) is a promising potential therapeutic strategy for diseases affecting myelin However, the derivation of engraftable OPCs from human pluripotent stem cells has proven difficult and primary OPCs are not readily available Here we report the generation of induced OPCs (iOPCs) by direct lineage conversion Forced expression of the three transcription factors Sox10, Olig2 and Zfp536 was sufficient to reprogram mouse and rat fibroblasts into iOPCs with morphologies and gene expression signatures resembling primary OPCs More importantly, iOPCs gave rise to mature oligodendrocytes that could ensheath multiple host axons when co-cultured with primary dorsal root ganglion cells and formed myelin after transplantation into shiverer mice We propose direct lineage reprogramming as a viable alternative approach for the generation of OPCs for use in disease modeling and regenerative medicineread more
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Protocol to isolate a large amount of functional oligodendrocyte precursor cells from the cerebral cortex of adult mice and humans.
TL;DR: A protocol to obtain viable adult OPCs from the cerebral cortex of different mouse strains is designed and compared with other well-known methods and it is shown that this protocol is also useful to isolate functional O PCs from human brain biopsies.
Journal ArticleDOI
Decoding cell signalling and regulation of oligodendrocyte differentiation.
Anderson K. Santos,Mariana S. Vieira,Rebecca Vasconcellos,Vânia Aparecida Mendes Goulart,Alexandre Hiroaki Kihara,Rodrigo R. Resende +5 more
TL;DR: These aspects, based on the most recent in vivo and in vitro data on oligodendrocyte generation and differentiation, are reviewed to understand the role of oligodendedrocytes in myelination in pathologies and normal conditions.
Journal ArticleDOI
Engineering Biomaterials with Micro/Nanotechnologies for Cell Reprogramming
Jun Fang,Yuan Yu Hsueh,Yuan Yu Hsueh,Jennifer Soto,Wujin Sun,Jinqiang Wang,Zhen Gu,Ali Khademhosseini,Song Li +8 more
TL;DR: An overview of the state-of-the-art technologies for cell reprograming is presented and the recent breakthroughs in engineering biomaterials with micro/nanotechnologies to improve reprogramming efficiency and quality are highlighted.
Journal ArticleDOI
Efficient conversion of human induced pluripotent stem cells into microglia by defined transcription factors.
Shih Wei Chen,Yu Sheng Hung,Jong Ling Fuh,Nien-Jung Chen,Yeh Shiu Chu,Shu Cian Chen,Ming-Ji Fann,Yu Hui Wong +7 more
TL;DR: In this article, the differentiation of human induced pluripotent stem cells (hiPSCs) into microglia (iMG) by forced expression of both SPI1 and CEBPA was reported.
Journal ArticleDOI
Stem cell therapies for age-related macular degeneration: the past, present, and future.
Yalong Dang,Chun Zhang,Yu Zhu +2 more
TL;DR: Recent advances in cell transplantation for dry AMD are summarized and obstacles and prospects in this field will also be discussed.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Cluster analysis and display of genome-wide expression patterns
TL;DR: A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression, finding in the budding yeast Saccharomyces cerevisiae that clustering gene expression data groups together efficiently genes of known similar function.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
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