Genome assembly of Chryseobacterium sp. strain IHBB 10212 from glacier top-surface soil in the Indian trans-Himalayas with potential for hydrolytic enzymes
Reads0
Chats0
TLDR
Several genes encoding the hydrolysis of ester linkages of triglycerides into fatty acids and glycerol were predicted in the genome and the annotation also predicted the genes encoding proteases, lipases, amylases, β-glucosidases, endoglucanases and xylanases involved in biotechnological processes.Abstract:
The cold-active esterases are gaining importance due to their catalytic activities finding applications in chemical industry, food processes and detergent industry as additives, and organic synthesis of unstable compounds as catalysts. In the present study, the complete genome sequence of 4,843,645 bp with an average 34.08% G + C content and 4260 protein-coding genes are reported for the low temperature-active esterase-producing novel strain of Chrysobacterium isolated from the top-surface soil of a glacier in the cold deserts of the Indian trans-Himalayas. The genome contained two plasmids of 16,553 and 11,450 bp with 40.54 and 40.37% G + C contents, respectively. Several genes encoding the hydrolysis of ester linkages of triglycerides into fatty acids and glycerol were predicted in the genome. The annotation also predicted the genes encoding proteases, lipases, amylases, β-glucosidases, endoglucanases and xylanases involved in biotechnological processes. The complete genome sequence of Chryseobacterium sp. strain IHBB 10212 and two plasmids have been deposited vide accession numbers CP015199, CP015200 and CP015201 at DDBJ/EMBL/GenBank.read more
Citations
More filters
Journal ArticleDOI
Bioelectrochemical vs hydrogenophilic approach for CO2 reduction into methane and acetate
Marco Zeppilli,Habib Chouchane,Laura Scardigno,Mouna Mahjoubi,Manuel Gacitúa,Refka Askri,Ameur Cherif,Mauro Majone +7 more
TL;DR: In this paper, the optimal pH has been selected through methane and acetate hydrogenophilic tests at three different pH values (5, 6.5, 7.5) while three different cathodic potentials (-0.7, −0.9, −1.1") have been tested in bioelectrochemical experiments by using the optimum pH value.
Journal ArticleDOI
Industrial applications of cold-adapted enzymes: challenges, innovations and future perspective.
TL;DR: In this paper, the authors highlight the significance of omics technology in enzyme bioprospection from extreme environments and highlight the challenges in using cold-adapted enzymes at the industrial level and the innovations associated with novel enzyme prospection strategies.
Journal ArticleDOI
Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
Antonio Rubio-del-Campo,Cristina Alcántara,Maria Carmen Collado,Jesús Rodríguez-Díaz,María J. Yebra +4 more
TL;DR: The results presented here indicated that each, LNB, GNB and 3FN disaccharide might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
Journal ArticleDOI
Recent Advances in Feedstock and Lipase Research and Development towards Commercialization of Enzymatic Biodiesel
TL;DR: In this paper, the authors reviewed the recent progress in feedstock development, including first, second, third, and fourth generation feedstocks for biodiesel production, and discussed recent progress of lipase research and development as one of the key factors for establishing a cost-competitive biodiesel process in terms of enzyme sources, properties, immobilization, and transesterification efficiency.
Journal ArticleDOI
Chryseobacterium glaciei sp. nov., isolated from the surface of a glacier in the Indian trans-Himalayas.
Mohinder Pal,Munesh Kumari,Shashi Kiran,Richa Salwan,Shanmugam Mayilraj,Sanjay Chhibber,Arvind Gulati +6 more
TL;DR: A novel bacterial strain isolated from a glacier near the Kunzum Pass located in the Lahaul-Spiti in the North-Western Himalayas of India is identified as a new species, for which the proposed name is Chryseobacterium glaciei sp.
References
More filters
Journal ArticleDOI
The RAST Server: Rapid Annotations using Subsystems Technology
Ramy K. Aziz,Ramy K. Aziz,Daniela Bartels,Aaron A. Best,Matthew DeJongh,Terrence Disz,Terrence Disz,Robert Edwards,Kevin Formsma,Svetlana Gerdes,Elizabeth M. Glass,Michael Kubal,Folker Meyer,Folker Meyer,Gary J. Olsen,Gary J. Olsen,Robert Olson,Robert Olson,Andrei L. Osterman,Ross Overbeek,Leslie Klis McNeil,Daniel Paarmann,Tobias Paczian,Bruce Parrello,Gordon D. Pusch,Claudia I. Reich,Rick Stevens,Rick Stevens,Olga Vassieva,Veronika Vonstein,Andreas Wilke,Olga Zagnitko +31 more
TL;DR: A fully automated service for annotating bacterial and archaeal genomes that identifies protein-encoding, rRNA and tRNA genes, assigns functions to the genes, predicts which subsystems are represented in the genome, uses this information to reconstruct the metabolic network and makes the output easily downloadable for the user.
Journal ArticleDOI
Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data
Chen-Shan Chin,David Alexander,Patrick Marks,Aaron Klammer,James P Drake,Cheryl Heiner,Alicia Clum,Alex Copeland,John Huddleston,Evan E. Eichler,Stephen Turner,Jonas Korlach +11 more
TL;DR: This work presents a hierarchical genome-assembly process (HGAP) for high-quality de novo microbial genome assemblies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing.
Journal ArticleDOI
WebMGA: a customizable web server for fast metagenomic sequence analysis
TL;DR: WebMGA, a customizable web server for fast metagenomic analysis that includes over 20 commonly used tools such as ORF calling, sequence clustering, quality control of raw reads, removal of sequencing artifacts and contaminations, taxonomic analysis, functional annotation etc.
Journal ArticleDOI
Interactive microbial genome visualization with GView
TL;DR: GView is a Java application for viewing and examining prokaryotic genomes in a circular or linear context that accepts standard sequence file formats and an optional style specification file to generate customizable, publication quality genome maps in bitmap and scalable vector graphics formats.
Journal ArticleDOI
Characterization of a new keratinolytic bacterium that completely degrades native feather keratin.
TL;DR: A novel feather-degrading microorganism was isolated from poultry waste, producing a high keratinolytic activity when cultured on broth containing native feather, and complete feather degradation was achieved during cultivation.