Human arthroplasty derived macrophages differentiate into osteoclastic bone resorbing cells
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TLDR
This is the first report showing that human macrophages isolated directly from periprosthetic tissues surrounding loosened implants can differentiate into multinucleated cells showing all the functional and cytochemical characteristics of osteoclasts.Abstract:
Objective—In aseptic loosening, a heavy macrophage response to biomaterial wear particles is commonly found in arthroplasty tissues. The aim of this study was to discover if these cells contribute to the bone resorption of aseptic loosening by diVerentiating into osteoclasts. Methods—Macrophages were isolated from the pseudocapsule and pseudomembrane of loose cemented and uncemented hip arthroplasties at the time of revision surgery and then co-cultured on glass coverslips and dentine slices with UMR 106 rat osteoblast-like cells, both in the presence and absence of 1,25 dihydroxyvitamin D3 [1,25(OH)2D3]. Macrophages isolated from the synovial membrane of patients with osteoarthritis (OA) undergoing hip replacements were similarly studied as a control group.read more
Citations
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Journal ArticleDOI
The Cellular and Molecular Biology of Periprosthetic Osteolysis
TL;DR: There are no approved treatments for osteolysis despite the promise of therapeutic agents against proinflammatory mediators and osteoclasts shown in animal models, and considerable efforts are underway to develop such therapies.
Journal ArticleDOI
Proposal for a histopathological consensus classification of the periprosthetic interface membrane
Lars Morawietz,R.A. Classen,J. H. Schröder,C. Dynybil,Carsten Perka,A. Skwara,J. Neidel,T. Gehrke,L. Frommelt,T. Hansen,Mike Otto,B. Barden,T. Aigner,P. Stiehl,T. Schubert,C. Meyer-Scholten,A. König,P. Ströbel,C. P. Rader,S. Kirschner,F. Lintner,W. Rüther,I. Bos,C. Hendrich,Jörg Kriegsmann,Veit Krenn +25 more
TL;DR: The classification proposed enables standardised typing of periprosthetic membranes and may serve as a tool for further research on the pathogenesis of the loosening of total joint replacement.
Journal ArticleDOI
The Central Role of Wear Debris in Periprosthetic Osteolysis
TL;DR: Recent advances in understanding of how wear debris causes osteolysis are reviewed, and emergent strategies for the avoidance and treatment of this disease are reviewed.
Journal ArticleDOI
Adherent Endotoxin on Orthopedic Wear Particles Stimulates Cytokine Production and Osteoclast Differentiation
Yanming Bi,Joscelyn M. Seabold,Scott G. Kaar,Ashraf A. Ragab,Victor M. Goldberg,James M. Anderson,Edward M. Greenfield +6 more
TL;DR: Results show that adherent endotoxin is involved in many of the biological responses induced by orthopedic wear particles and should stimulate development of new approaches designed to reduce the activity of adherent endot toxin in patients with Orthopedic implants.
References
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The problem is osteolysis
TL;DR: The thesis that osteolysis is the dominant problem in total hip arthroplasty is supported by observations that suggest that periprosthetic osteolytics is the leading problem in contemporary total hip replacement.
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