Open Access
Identification of small molecules for human hepatocyte expansion and iPS differentiation
Jing Shan,Nathan T. Ross,David J. Logan,David Thomas,Stephen A. Duncan,Trista E. North,Wolfram Goessling,Anne E. Carpenter,Robert E. Schwartz,Sangeeta N. Bhatia +9 more
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TLDR
A high-throughput screening platform for primary human hepatocytes is developed to identify small molecules in two different classes that can be used to generate renewable sources of functional human liver cells in vitro.Abstract:
Broad Institute of MIT and Harvard (Scientific Planning and Allocation of Resources Committee Grant)read more
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Human induced pluripotent stem cell-derived hepatocytes for toxicology testing.
TL;DR: Combining iPSC-HCs with organotypic culture systems affords an opportunity to maximize the potential of both technologies where the cells benefit from more complex culture conditions while unlocking the Potential of the culture systems by affording stability and reproducibility to provide the future of predictive in vitro toxicity models.
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The Role of Microfluidics for Organ on Chip Simulations
TL;DR: In this review, recent progress regarding microfluidic devices and their applications in cell cultures is discussed and future challenges regarding the simulation of OCs are discussed to explain the advantages and limitations of these systems.
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A Comparative Assessment of Human and Chimpanzee iPSC-derived Cardiomyocytes with Primary Heart Tissues.
TL;DR: It is determined that gene expression in cultured cardiomyocytes from both human and chimpanzee is most similar to that of adult hearts compared to other adult tissues, and inter-species regulatory differences seen in carduomyocytes are found significantly more often in hearts than in other primary tissues.
Journal ArticleDOI
3-D culture and endothelial cells improve maturity of human pluripotent stem cell-derived hepatocytes.
Hamisha Ardalani,Srikumar Sengupta,Victoria Harms,Vernella Vickerman,James A. Thomson,William L. Murphy +5 more
TL;DR: It is shown that 3D culture of iPS-derived hepatocytes and their co-culture with human sinusoidal endothelial cells (sECs) to improve their maturity is shown, suggesting that the iHEP/EC aggregates described here may have the potential to be used for many applications, including as an in vitro model to study liver diseases associated with sinusoid endothelial Cells.
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Stem cell–derived models to improve mechanistic understanding and prediction of human drug-induced liver injury
Christopher E. Goldring,Daniel J. Antoine,Frank Bonner,Jonathan Crozier,Chris Denning,Robert J. Fontana,Neil A. Hanley,David C. Hay,Magnus Ingelman-Sundberg,Satu Juhila,Neil R. Kitteringham,Beatriz Silva-Lima,Alan Norris,Chris S. Pridgeon,James A. Ross,Rowena Sison Young,Danilo Tagle,Belen Tornesi,Bob van de Water,Richard J. Weaver,Fang Zhang,B. Kevin Park +21 more
TL;DR: The importance of benchmarking stem cell–derived hepatocyte‐like cells to their terminally differentiated human counterparts using defined phenotyping is discussed, to make sure the cells are relevant and comparable between labs, and outline why this process is essential before the cells is introduced into chemical safety assessment.
References
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Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Global epidemiology of hepatitis C virus infection
TL;DR: Because there is no vaccine and no post-exposure prophylaxis for HCV, the focus of primary prevention efforts should be safer blood supply in the developing world, safe injection practices in health care and other settings, and decreasing the number of people who initiate injection drug use.
Journal Article
Liver regeneration : Frontiers in medicine: Regeneration
TL;DR: This review attempts to integrate the findings of the last three decades and looks toward clues as to the nature of the causes that trigger this fascinating organ and cellular response.
Genome sequence of the Brown Norway rat yields insights into mammalian evolutionRat Genome Sequencing Project ConsortiumNature200442849352115057822
Richard A. Gibbs,George M. Weinstock,Michael L. Metzker,Donna M. Muzny,Erica Sodergren,Steven E. Scherer,Graham R. Scott,David Steffen,Kim C. Worley,Paula E. Burch,Geoffrey Okwuonu,Sandra Hines,Lora Lewis,C. DeRamo,Oliver Delgado,S. Dugan Rocha,George Miner,Margaret Morgan,Alicia Hawes,Rachel Gill,Robert A. Holt,Mark Raymond Adams,Peter G. Amanatides,H. Baden Tillson,Mary Barnstead,Soo H. Chin,Cheryl A. Evans,Steve Ferriera,Carl Fosler,A. Glodek,Gu Zp,D. Jennings,Cheryl L. Kraft,T. Nguyen,Cynthia Pfannkoch,Cynthia Sitter,Granger G. Sutton,J. C. Venter,Trevor Woodage,Douglas Smith,H. M. Lee,Erik Gustafson,Patrick Cahill,A. Kana,L. Doucette Stamm,Keith Weinstock,Kim Fechtel,Robert B. Weiss,Diane M. Dunn,Eric D. Green,Robert W. Blakesley,Gerard G. Bouffard,P. J. De Jong,Kazutoyo Osoegawa,Zhu Bl,Marco A. Marra,Jacqueline E. Schein,Ian Bosdet,Chris Fjell,Steven J.M. Jones,Martin Krzywinski,Carrie Mathewson,Asim Sarosh Siddiqui,Natasja Wye,John Douglas Mcpherson,Zhao Sy,Claire M. Fraser,Jyoti Shetty,Sofiya Shatsman,Keita Geer,Chen Yx,S. Abramzon,William C. Nierman,Paul Havlak,Rui Chen,K. J. Durbin,Amy Egan,Ren Yr,Xing Zhi Song,Li Bs,Yue Liu,Xiang Qin,Simon Cawley,Austin J. Cooney,Lisa M. D'Souza,Kirt Martin,Jia Qian Wu,M. L. Gonzalez Garay,Andrew R. Jackson,K. J. Kalafus,Michael P. McLeod,Aleksandar Milosavljevic,D. Virk,A. Volkov,David A. Wheeler,Zhang Zd,Jeffrey A. Bailey,Evan E. Eichler,Eray Tüzün,Ewan Birney,Emmanuel Mongin,A. Ureta Vidal,C. Woodwark,Evgeny M. Zdobnov,Peer Bork,Mikita Suyama,David Torrents,Marina Alexandersson,Barbara J. Trask,Janet M. Young,Hui Huang,Wang Hj,Xing Hm,Sue Daniels,Darryl Gietzen,Jeanette Schmidt,K. Stevens,Ursula Vitt,Jim Wingrove,Francisco Camara,M. Mar Albà,Josep F. Abril,Roderic Guigó,Arian F.A. Smit,Inna Dubchak,Edward M. Rubin,Olivier Couronne,Alexander Poliakov,Norbert Hubner,Detlev Ganten,C. Goesele,Oliver Hummel,Thomas Kreitler,Young-Ae Lee,Jan Monti,Herbert Schulz,Heike Zimdahl,Heinz Himmelbauer,Hans Lehrach,Howard J. Jacob,Susan Bromberg,J. Gullings Handley,M. I. Jensen Seaman,Anne E. Kwitek,Jozef Lazar,Dean Pasko,Peter J. Tonellato,Simon N. Twigger,Chris P. Ponting,Jose M. Duarte,Stephen Rice,Leo Goodstadt,Scott A. Beatson,Richard D. Emes,Eitan E. Winter,Caleb Webber,Petra Brandt,Gerald Nyakatura,M. Adetobi,Francesca Chiaromonte,Laura Elnitski,Pallavi Eswara,Ross C. Hardison,Hou Mm,Diana L. Kolbe,Kateryna D. Makova,Webb Miller,Anton Nekrutenko,Cathy Riemer,Scott Schwartz,James Taylor,Shan Yang,Yi Zhang,Klaus Lindpaintner,T. D. Andrews,Mario Caccamo,Michele Clamp,Laura Clarke,V. Curwen,Richard Durbin,Eduardo Eyras,Stephen M. J. Searle,Gregory M. Cooper,Serafim Batzoglou,Michael Brudno,Arend Sidow,Eric A. Stone,Bret A. Payseur,Guillaume Bourque,C. Lopez Otin,Xose S. Puente,Kushal Chakrabarti,Sourav Chatterji,Colin N. Dewey,Lior Pachter,Nicolas Bray,Von Bing Yap,Anat Caspi,G. Tesler,Pavel A. Pevzner,David Haussler,Krishna M. Roskin,Robert Baertsch,Hiram Clawson,Terrence S. Furey,Angie S. Hinrichs,Donna Karolchik,W. J. Kent,Kate R. Rosenbloom,Heather Trumbower,M. Weirauch,David Neil Cooper,Peter D. Stenson,Bin Ma,Michael R. Brent,Manimozhiyan Arumugam,David Shteynberg,Richard R. Copley,Martin S. Taylor,Harold Riethman,Uma Mudunuri,Jane Peterson,Mark S. Guyer,Adam Felsenfeld,Susan Old,Stephen C. Mockrin,Francis S. Collins +226 more
Abstract: The laboratory rat (Rattus norvegicus) is an indispensable tool in experimental medicine and drug development, having made inestimable contributions to human health. We report here the genome sequence of the Brown Norway (BN) rat strain. The sequence represents a high-quality ‘draft’ covering over 90% of the genome. The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution.
Journal ArticleDOI
Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo
Evert Kroon,Laura Martinson,Kuniko Kadoya,Anne G. Bang,Olivia Kelly,Susan Eliazer,Holly Y. Young,Mike Richardson,Nora G. Smart,Justine J Cunningham,Alan D. Agulnick,Kevin A. D'Amour,Melissa K. Carpenter,Emmanuel E. Baetge +13 more
TL;DR: It is shown that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice, and it is demonstrated that implantation of hES cell–derived pancreaticEndoderm protects against streptozotocin-induced hyperglycemia.