Imaging of nucleic acids with atomic force microscopy
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TLDR
Recent advances with the use of time-lapse AFM are reviewed, with emphasis on methods utilizing modification of mica to prepare the surfaces enabling reliable and reproducible imaging of DNA and RNA nanostructures.About:
This article is published in Methods.The article was published on 2011-06-01 and is currently open access. It has received 158 citations till now. The article focuses on the topics: DNA nanotechnology.read more
Citations
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Studying non-covalent drug-DNA interactions.
TL;DR: This review focuses on discussing various techniques used to study non-covalent interactions that occur between drugs and DNA and reports several techniques that may be employed to analyse the binding mode of a drug with DNA.
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Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy
TL;DR: The protocols are adaptable in general for imaging many proteins and protein–nucleic acid complexes, and examples are described for looking at walking myosin, ATP-hydrolyzing rotorless F1-ATPase and cellulose-hydrosin, and the entire protocol takes 10–15 h, depending mainly on the substrate surface to be used.
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Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology.
TL;DR: The application of RNA nanotechnology for specific and efficient delivery of anti-miR-21 to block the growth of TNBC in orthotopic mouse models is reported here and demonstrates the clinical potentials ofRNA nanotechnology based platform to deliver miRNA based therapeutics for cancer treatment.
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Design and development of nanosized DNA assemblies in polypod-like structures as efficient vehicles for immunostimulatory CpG motifs to immune cells.
Kohta Mohri,Makiya Nishikawa,Natsuki Takahashi,Tomoki Shiomi,Nao Matsuoka,Kohei Ogawa,Masayuki Endo,Kumi Hidaka,Hiroshi Sugiyama,Yuki Takahashi,Yoshinobu Takakura +10 more
TL;DR: The results indicate that CpG DNA-containing polypodna preparations with six or more pods are a promising nanosized device with biodegradability and high immunostimulatory activity.
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Allele-Specific Reprogramming of Cancer Metabolism by the Long Non-coding RNA CCAT2
Roxana S. Redis,Luz E. Vela,Weiqin Lu,Juliana Ferreira de Oliveira,Cristina Ivan,Cristian Rodriguez-Aguayo,Douglas Adamoski,Barbara Pasculli,Ayumu Taguchi,Yunyun Chen,Agustín F. Fernández,Luis Valledor,Katrien Van Roosbroeck,Samuel Chang,Maitri Y. Shah,Garrett Kinnebrew,Leng Han,Yaser Atlasi,Lawrence H. Cheung,Gilbert Yuanjay Huang,Paloma Monroig,Marc S. Ramirez,Tina Catela Ivković,Long Van,Hui Ling,Roberta Gafà,Sanja Kapitanović,Giovanni Lanza,James A. Bankson,Peng Huang,Stephan Y. Lai,Robert C. Bast,Michael G. Rosenblum,Milan Radovich,Mircea Ivan,Geoffrey Bartholomeusz,Han Liang,Mario F. Fraga,William R. Widger,Samir M. Hanash,Ioana Berindan-Neagoe,Ioana Berindan-Neagoe,Ioana Berindan-Neagoe,Gabriel Lopez-Berestein,Andre Luis Berteli Ambrosio,Sandra Martha Gomes Dias,George A. Calin +46 more
TL;DR: The CCAT2 interaction with the CFIm complex fine-tunes the alternative splicing of Glutaminase (GLS) by selecting the poly(A) site in intron 14 of the precursor mRNA.
References
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A high-speed atomic force microscope for studying biological macromolecules.
TL;DR: In this paper, a high-speed scanner, free of resonant vibrations up to 60 kHz, small cantilevers with high resonance frequencies (450-650 kHz) and small spring constants (150-280 pN/nm), and several electronic devices of wide bandwidth are presented.
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Video imaging of walking myosin V by high-speed atomic force microscopy
TL;DR: This work directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy, providing corroborative ‘visual evidence’ for previously speculated or demonstrated molecular behaviours, and reveals more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism.
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The emerging field of RNA nanotechnology
TL;DR: In this paper, the authors review techniques for constructing RNA nanoparticles from different building blocks, describe the distinct attributes of RNA inside the body, and discuss potential applications of RNA nanostructures in medicine.
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Short cantilevers for atomic force microscopy
D. A. Walters,Jason Cleveland,Neil H. Thomson,Paul K. Hansma,Mark A. Wendman,G. Gurley,V. Elings +6 more
TL;DR: In this paper, a family of silicon nitride cantilevers ranging in length from 23 to 203 μm were designed and tested, and the frequency spectrum of thermal motion in air and water was measured.
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Building Programmable Jigsaw Puzzles with RNA
Arkadiusz Chworos,Isil Severcan,Alexey Y. Koyfman,Patrick Weinkam,Emin Oroudjev,Helen G. Hansma,Luc Jaeger +6 more
TL;DR: This work achieves reliable prediction and design of the three-dimensional structure of artificial RNA building blocks to generate molecular jigsaw puzzle units called tectosquares and demonstrates that fully addressable materials based on RNA can be synthesized.