Integration of Ultra-Low Volume Pneumatic Microfluidics with a Three-Dimensional Electrode Network for On-Chip Biochemical Sensing.
TLDR
In this paper, a miniaturized pseudo reference electrode (RE) design for biasing ion sensitive field effect transistors (ISFETs) is presented. But the pseudo-RE does not have the ability to scale up in numbers with the CMOS scaling.Abstract:
This paper reports a novel miniaturized pseudo reference electrode (RE) design for biasing Ion Sensitive Field Effect Transistors (ISFETs). It eliminates the need for post-CMOS processing and can scale up in numbers with the CMOS scaling. The presented design employs silane-mediated transfer of patterned gold electrode lines onto PDMS microfluidics such that the gold conformally coats the inside of microfluidic channel. Access to this electrode network is made possible by using “through-PDMS-vias” (TPV), which consist of high metal-coated SU-8 pillars manufactured by a novel process that employs a patterned positive resist layer as SU-8 adhesion depressor. When integrated with pneumatic valves, TPV and pseudo-RE network were able to bias 1.5 nanoliters (nL) of isolated electrolyte volumes. We present a detailed characterization of our pseudo-RE design demonstrating ISFET operation and its DC characterization. The stability of pseudo-RE is investigated by measuring open circuit potential (OCP) against a commercial Ag/AgCl reference electrode.read more
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Thermodynamic Characterization of a Highly Transparent Microfluidic Chip with Multiple On-Chip Temperature Control Units
TL;DR: In this paper , a multilayer transparent microfluidic chip with multiple sets of on-chip heating, local temperature measurement and positive onchip cooling function units was designed and fabricated.
References
More filters
Journal ArticleDOI
Monolithic microfabricated valves and pumps by multilayer soft lithography
TL;DR: An extension to the soft lithography paradigm, multilayersoft lithography, with which devices consisting of multiple layers may be fabricated from soft materials is described, to build active microfluidic systems containing on-off valves, switching valves, and pumps entirely out of elastomer.
Journal ArticleDOI
Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study.
Craig A Poland,Rodger Duffin,Ian A. Kinloch,Andrew D. Maynard,William A Wallace,Anthony Seaton,Vicki Stone,Simon Brown,William MacNee,Ken Donaldson +9 more
TL;DR: Exposing the mesothelial lining of the body cavity of mice to long multiwalled carbon nanotubes results in asbestos-like, length-dependent, pathogenic behaviour, including inflammation and the formation of lesions known as granulomas.
Journal ArticleDOI
An integrated semiconductor device enabling non-optical genome sequencing
Jonathan M. Rothberg,Wolfgang Hinz,Todd Rearick,Jonathan Schultz,William J. Mileski,Melville Davey,John H. Leamon,Kim L. Johnson,Mark James Milgrew,Matthew D. Edwards,Jeremy Hoon,Jan Fredrik Simons,David Marran,Jason W. Myers,John F. Davidson,Annika Branting,John Nobile,Bernard P. Puc,David Light,Travis A. Clark,Martin Huber,Jeffrey T. Branciforte,Isaac B. Stoner,Simon Cawley,Michael R. Lyons,Yutao Fu,Nils Homer,Marina Sedova,Xin Miao,Brian Reed,Jeffrey Sabina,Erika Feierstein,Michelle Schorn,Mohammad Alanjary,Eileen T. Dimalanta,Devin Dressman,Rachel Kasinskas,Tanya Sokolsky,Jacqueline A. Fidanza,Eugeni Namsaraev,Kevin McKernan,Alan Williams,G. Thomas Roth,James Bustillo +43 more
TL;DR: A DNA sequencing technology in which scalable, low-cost semiconductor manufacturing techniques are used to make an integrated circuit able to directly perform non-optical DNA sequencing of genomes, showing its robustness and scalability by producing ion chips with up to 10 times as many sensors and sequencing a human genome.
Journal ArticleDOI
Liquid Biopsies: Genotyping Circulating Tumor DNA
Luis A. Diaz,Alberto Bardelli +1 more
TL;DR: The ability to detect and quantify tumor mutations has proven effective in tracking tumor dynamics in real time as well as serving as a liquid biopsy that can be used for a variety of clinical and investigational applications not previously possible.
Journal ArticleDOI
Digital PCR
TL;DR: An approach for transforming the exponential, analog nature of the PCR into a linear, digital signal suitable for this purpose is described and demonstrated through the detection of a mutant ras oncogene in the stool of patients with colorectal cancer.
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