Journal ArticleDOI
Interrogating immune cells and cancer with CRISPR-Cas9.
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TLDR
A review of recent developments in CRISPR-Cas9 technology and how they have been leveraged to discover and manipulate novel genetic regulators of the immune system can be found in this article.About:
This article is published in Trends in Immunology.The article was published on 2021-05-01. It has received 8 citations till now.read more
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Journal ArticleDOI
The Role of Interferons in Melanoma Resistance to Immune Checkpoint Blockade: Mechanisms of Escape and Therapeutic Implications
TL;DR: In this article, a review highlights the current understanding of the mechanisms by which tumors disrupt interferon function in the context of immune checkpoint blockade, and discusses therapeutic strategies to overcome these mechanisms of resistance and improve the clinical reach of checkpoint blockade therapy in melanoma patients.
Journal ArticleDOI
Prospective approaches to enhancing CAR T cell therapy for glioblastoma
Sun Il Choi,Jinlong Yin +1 more
TL;DR: Effectively improved CAR T cells may offer patients with GBM new treatment opportunities, and this review is intended to provide a comprehensive overview for researchers to develop potentCAR T cells using genetic engineering or combinatorial preparations.
Posted ContentDOI
A novel Bayesian factor analysis method improves detection of genes and biological processes affected by perturbations in single-cell CRISPR screening
TL;DR: Guided Sparse Factor Analysis (GSFA) as discussed by the authors infers latent factors that represent co-regulated genes or gene modules, and by borrowing information from these factors, infers the effects of genetic perturbations on individual genes.
Journal ArticleDOI
High-content CRISPR screening in tumor immunology
Erin A Holcomb,Ashley N Pearson,Kassidy M. Jungles,Akshay Tate,Jadyn G. James,Long Yue Jiang,Amanda K. Huber,Michael D. Green +7 more
TL;DR: High-content CRISPR screening is a powerful tool that links specific genetic alterations to corresponding phenotypes, thus allowing for high-throughput identification of novel gene functions as mentioned in this paper .
Journal ArticleDOI
Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction
Ali A. Rabaan,Hajir Alsaihati,Rehab Bukhamsin,Muhammed A Bakhrebah,Majed S. Nassar,Abdulmonem A. Alsaleh,Yousef N. Alhashem,Ammar Y. Bukhamseen,Khalil Al-Ruhimy,M. Alotaibi,Roua A. Alsubki,Hejji E Alahmed,Saleh Al-Abdulhadi,Fatemah A. Alhashem,Ahlam Alqatari,Ahmed Alsayyah,R. Farahat,Rwaa H. Abdulal,Ali Hussein Ali Al-Ahmed,Mohd. Imran,Ranjan K. Mohapatra +20 more
TL;DR: Wang et al. as discussed by the authors reviewed CRISPR/Cas9 for tumor therapy research, which will be helpful in providing references for future studies on the pathogenesis of malignancy and its treatment.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
Evan Z. Macosko,Evan Z. Macosko,Anindita Basu,Anindita Basu,Rahul Satija,Rahul Satija,James Nemesh,James Nemesh,Karthik Shekhar,Melissa Goldman,Melissa Goldman,Itay Tirosh,Allison R. Bialas,Nolan Kamitaki,Nolan Kamitaki,Emily M. Martersteck,John J. Trombetta,David A. Weitz,Joshua R. Sanes,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Aviv Regev,Aviv Regev,Aviv Regev,Steven A. McCarroll,Steven A. McCarroll +26 more
TL;DR: Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together.
Journal ArticleDOI
Development and applications of CRISPR-Cas9 for genome engineering.
TL;DR: In this paper, the authors describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions, and highlight challenges and future directions.