Isolation of cancer stem cells from adult glioblastoma multiforme
Xiangpeng Yuan,James F. Curtin,Yizhi Xiong,Gentao Liu,Sebastian Waschsmann-Hogiu,Daniel L. Farkas,Keith L. Black,John S. Yu +7 more
TLDR
The identification of tumor stem cells within adult GBM may represent a major step forward in understanding the origin and maintenance of GBM and lead to the identification and testing of new therapeutic targets.Abstract:
Glioblastoma multiforme (GBM) is the most common adult primary brain tumor and is comprised of a heterogeneous population of cells. It is unclear which cells within the tumor mass are responsible for tumor initiation and maintenance. In this study, we report that brain tumor stem cells can be identified from adult GBMs. These tumor stem cells form neurospheres, possess the capacity for self-renewal, express genes associated with neural stem cells (NSCs), generate daughter cells of different phenotypes from one mother cell, and differentiate into the phenotypically diverse populations of cells similar to those present in the initial GBM. Having a distinguishing feature from normal NSCs, these tumor stem cells can reform spheres even after the induction of differentiation. Furthermore, only these tumor stem cells were able to form tumors and generate both neurons and glial cells after in vivo implantation into nude mice. The identification of tumor stem cells within adult GBM may represent a major step forward in understanding the origin and maintenance of GBM and lead to the identification and testing of new therapeutic targets.read more
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Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.
Jeongwu Lee,Svetlana Kotliarova,Yuri Kotliarov,Aiguo Li,Qin Su,Nicholas M. Donin,Sandra Pastorino,Benjamin Purow,Neil Christopher,Wei Zhang,John K. Park,Howard A. Fine +11 more
TL;DR: Significant phenotypic and genotypic differences are demonstrated between primary human tumor-derived TSCs and their matched glioma cell lines, suggesting that TSC's may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors.
Journal ArticleDOI
Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma.
Gentao Liu,Gentao Liu,Xiangpeng Yuan,Zhaohui Zeng,Patrizia Tunici,Hiushan Ng,Iman R. Abdulkadir,Lizhi Lu,Dwain K. Irvin,Keith L. Black,John S. Yu +10 more
TL;DR: This study for the first time provided evidence that CD133 positive cancer stem cells display strong capability on tumor's resistance to chemotherapy.
Journal ArticleDOI
Multicellular tumor spheroids: an underestimated tool is catching up again
Franziska Hirschhaeuser,Heike Menne,Claudia Dittfeld,Jonathan West,Wolfgang Mueller-Klieser,Leoni A. Kunz-Schughart +5 more
TL;DR: The rationale, potential and flexibility of tumor spheroid mono- and cocultures for implementation into state of the art anti-cancer therapy test platforms are highlighted and the relevance of the cancer stem cell hypothesis for cancer cure is highlighted.
Journal ArticleDOI
A tumorigenic subpopulation with stem cell properties in melanomas.
Douglas D. Fang,Thiennga K. Nguyen,Kim Leishear,Rena Finko,Angela N. Kulp,Susan Hotz,Patricia Van Belle,Xiaowei Xu,David E. Elder,Meenhard Herlyn +9 more
TL;DR: It is proposed that melanomas can contain a subpopulation of stem cells that contribute to heterogeneity and tumorigenesis, and targeting this population may lead to effective treatments for melanomas.
Journal ArticleDOI
CD133(+) and CD133(-) glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles.
Dagmar Beier,Peter Hau,Martin Proescholdt,Annette Lohmeier,Jörg Wischhusen,Peter J. Oefner,Ludwig Aigner,Alexander Brawanski,Ulrich Bogdahn,Christoph P. Beier +9 more
TL;DR: Together, the data provide first evidence that CD133(+) CSC maintain only a subset of primary glioblastomas, with apparent stem cell-like properties but distinct molecular profiles and growth characteristics in vitro and in vivo.
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