Ligation of CD40 on dendritic cells triggers production of high levels of interleukin-12 and enhances T cell stimulatory capacity: T-T help via APC activation.
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TLDR
It is found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12, which is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs.Abstract:
We investigated the possibility that T helper cells might enhance the stimulatory function of dendritic cells (DCs). We found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12. Other stimuli such as microbial agents, TNF-alpha or LPS are much less effective or not at all. In addition, CD40L is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs. These effects of CD40 ligation result in an increased capacity of DCs to trigger proliferative responses and IFN-gamma production by T cells. These findings reveal a new role for CD40-CD40L interaction in regulating DC function and are relevant to design therapeutic strategies using cultured DCs.read more
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Dendritic cell longevity and T cell persistence is controlled by CD154-CD40 interactions.
Amy J. Miga,Sally R. Masters,Brigit G. Durell,Mercedes Gonzalez,Marc K. Jenkins,Charles Maliszewski,Hitoshi Kikutani,William F. Wade,Randolph J. Noelle +8 more
TL;DR: Findings show that both T cell and DC persistence in vivo is dependent on CD40‐CD154 interactions, and the profound impact of CD154 deficiency on cell‐mediated immunity may be due to its ability to limit the duration of antigen presentation in vivo and cause the premature demise of antigen‐specific T cells.
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CD40-CD40 Ligand-Independent Activation of CD8+ T Cells Can Trigger Allograft Rejection
Nick D. Jones,A Van Maurik,Masaki Hara,Bernd M. Spriewald,O Witzke,Peter J. Morris,Kathryn J. Wood +6 more
TL;DR: It is concluded that CD40L is not an important costimulatory molecule for CD8+ T cell activation and that following transplantation donor APC can activate recipient CD8- T cells directly without first being primed by CD4+ T cells.
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Comparative Analysis of Genetically Modified Dendritic Cells and Tumor Cells as Therapeutic Cancer Vaccines
TL;DR: It is found that vaccination with bone marrow–derived DCs engineered to express MAGE-1 via adenoviral-mediated gene transfer led to a dramatic decrease in the number of metastases in a lung metastasis model, and led to prolonged survival and some long-term cures in a subcutaneous preexisting tumor model.
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Enhancement of Human Cord Blood CD34+ Cell-Derived NK Cell Cytotoxicity by Dendritic Cells
Ying Yu,Masao Hagihara,Kiyoshi Ando,Balgansuren Gansuvd,Hideyuki Matsuzawa,Takahide Tsuchiya,Yoko Ueda,Hiroyasu Inoue,Tomomitsu Hotta,Shunichi Kato +9 more
TL;DR: Two culture systems in which human cord blood CD34+ cells from the same donor were induced to generate NK cells and DCs suggest that DCs enhance NK cell cytotoxicity by up-regulating both perforin/granzyme B- and FasL/Fas-based pathways.
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A member of the dendritic cell family that enters B cell follicles and stimulates primary antibody responses identified by a mannose receptor fusion protein.
Claude Berney,Suzanne Herren,Christine A. Power,Siamon Gordon,Luisa Martinez-Pomares,Marie H. Kosco-Vilbois +5 more
TL;DR: Evidence is provided that a subset of DCs enters primary follicles, armed with the capacity to attract and provide antigenic stimulation for T and B lymphocytes, after a primary immunization.
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