Ligation of CD40 on dendritic cells triggers production of high levels of interleukin-12 and enhances T cell stimulatory capacity: T-T help via APC activation.
Reads0
Chats0
TLDR
It is found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12, which is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs.Abstract:
We investigated the possibility that T helper cells might enhance the stimulatory function of dendritic cells (DCs). We found that ligation of CD40 by CD40L triggers the production of extremely high levels of bioactive IL-12. Other stimuli such as microbial agents, TNF-alpha or LPS are much less effective or not at all. In addition, CD40L is the most potent stimulus in upregulating the expression of ICAM-1, CD80, and CD86 molecules on DCs. These effects of CD40 ligation result in an increased capacity of DCs to trigger proliferative responses and IFN-gamma production by T cells. These findings reveal a new role for CD40-CD40L interaction in regulating DC function and are relevant to design therapeutic strategies using cultured DCs.read more
Citations
More filters
Journal ArticleDOI
Enhancement of dendritic cell activation via CD40 ligand-expressing γδ T cells is responsible for protective immunity to Plasmodium parasites
Shin-Ichi Inoue,Mamoru Niikura,Satoru Takeo,Shoichiro Mineo,Yasushi Kawakami,Akihiko Uchida,Shigeru Kamiya,Fumie Kobayashi +7 more
TL;DR: It is found that γδ T cells play a key role in dendritic cell activation after Plasmodium infection and are supported strongly by the fact that in vivo induction of CD40 signaling prevented the death of TCRδ-KO mice after infection with P. berghei XAT.
Journal ArticleDOI
High Expression of Antioxidant Proteins in Dendritic Cells Possible Implications in Atherosclerosis
Aymeric Rivollier,Laure Perrin-Cocon,Sylvie Luche,Hélène Diemer,Jean-Marc Strub,Daniel Hanau,Alain Van Dorsselaer,Vincent Lotteau,Chantal Rabourdin-Combe,Thierry Rabilloud,Christine Servet-Delprat +10 more
TL;DR: In conclusion, DCs were more resistant than monocytes to apoptosis induced by high amounts of oxidized low density lipoproteins containing toxic organic peroxides and hydrogen peroxide, and DC-stimulated T cells produced high levels of receptor activator of nuclear factor κB ligand, a chemotactic and survival factor for monocytes and DCs.
Journal ArticleDOI
Characterization of the phosphodiesterase (PDE) pattern of in vitro-generated human dendritic cells (DC) and the influence of PDE inhibitors on DC function.
TL;DR: PDE inhibitors can affect DC function directly as demonstrated by blocking TNF release and their efficacy reflects the changes in the PDE activity profile during differentiation from their monocyte precursors.
Journal ArticleDOI
Activation-induced non-responsiveness (anergy) limits CD8 T cell responses to tumors.
Matthew F. Mescher,Flavia E. Popescu,Michael Y. Gerner,Chris D. Hammerbeck,Julie M. Curtsinger +4 more
TL;DR: The activation-induced non-responsiveness (AINR) as discussed by the authors is a form of anergy characterized by an inability to produce IL-2, which limits the ability of activated CTL to control tumor growth, but can be reversed by activating either therapeutically or by activated CD4 T helper cells to allow continued expansion.
Journal ArticleDOI
Dendritic cells in rheumatoid arthritis.
Sujata Sarkar,David A. Fox +1 more
TL;DR: Dendritic cells may be central to the pathogenesis of RA and could also be logical targets for treatment, and DC s themselves could be used to deliver therapeutic gene products in autoimmune disease.
References
More filters
Journal ArticleDOI
Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.
TL;DR: Cultured DCs are as efficient as antigen-specific B cells in presenting tetanus toxoid (TT) to specific T cell clones and their efficiency of antigen presentation can be further enhanced by specific antibodies via FcR- mediated antigen uptake.
Journal ArticleDOI
The dendritic cell system and its role in immunogenicity
TL;DR: Dendritic cells are specialized to mediate several physiologic components of immunogenicity such as the acquisition of antigens in tissues, the migration to lymphoid organs, and the identification and activation of antigen-specific T cells.
Journal ArticleDOI
Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages
Chyi-Song Hsieh,Steven E. Macatonia,Catherine S. Tripp,Stanley F. Wolf,Anne O'Garra,Kenneth M. Murphy +5 more
TL;DR: This regulatory pathway may have evolved to enable innate immune cells, through interactions with microbial pathogens, to direct development of specific immunity toward the appropriate TH1 phenotype.
Journal ArticleDOI
Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products.
TL;DR: The capacity of DCs to capture and process antigen could be modulated by exogenous stimuli was investigated and it was found that DCs respond to tumor necrosis factor alpha, CD40 ligand, IL-1, and lipopolysaccharide with a coordinate series of changes that include downregulation of macropinocytosis and Fc receptors, disappearance of the class II compartment, and upregulation of adhesion and costimulatory molecules.
Journal ArticleDOI
Natural killer cell stimulatory factor (interleukin 12 [IL-12]) induces T helper type 1 (Th1)-specific immune responses and inhibits the development of IL-4-producing Th cells.
Roberto Manetti,Paola Parronchi,Maria Grazia Giudizi,Marie-Pierre Piccinni,Enrico Maggi,Giorgio Trinchieri,Sergio Romagnani +6 more
TL;DR: IL-12 and CD16+ cells appear to have inhibitory effects on the development of IL-4-producing cells and to play an inductive role in promoting Th1-like responses.