Journal ArticleDOI
LYK4 is a component of a tripartite chitin receptor complex in Arabidopsis thaliana
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TLDR
It is suggested that LyK4 functions as a LYK5-associated co-receptor or scaffold protein that enhances chitin-induced signaling in A. thaliana.Abstract:
LysM receptor-like kinases (LYKs) of Arabidopsis thaliana (namely LYK1, LYK4 and LYK5) play a major role in chitin perception and immunity against pathogenic fungi. Chitin-induced heterodimerization of LYK1 and LYK5 has been previously reported, but protein interaction partners of LYK4 have not yet been identified. In this study, by analysing mutants we confirmed a role of LYK4 in chitin perception, and found that the ectodomain of LYK4 homodimerizes and also interacts with the ectodomain of LYK5 in vitro. Pull-down experiments with proteins expressed in protoplasts indicated LYK4-LYK4 and LY4-LYK5 interactions in planta. When protoplasts were treated with chitoheptaose or chitin, a protein complex was immunoprecipitated that appeared to be composed of LYK1, LYK4, and LYK5. Similar experiments with proteins expressed in lyk mutant plants suggested that elicitor treatment induced a physical interaction between LYK1 and LYK5 but not between LYK1 and LYK4. Bimolecular fluorescence complementation experiments substantiated these findings. Overall, our data suggest that LYK4 functions as a LYK5-associated co-receptor or scaffold protein that enhances chitin-induced signaling in Arabidopsis.read more
Citations
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Journal ArticleDOI
Surface Sensor Systems in Plant Immunity.
Isabell Albert,Chenlei Hua,Thorsten Nürnberger,Thorsten Nürnberger,Rory N Pruitt,Lisha Zhang +5 more
TL;DR: Protein complexes at the cell surface facilitate the detection of danger signals from diverse pathogens and initiate a series of complex intracellular signaling events that result in various immune responses.
Journal ArticleDOI
Hide-and-Seek: Chitin-Triggered Plant Immunity and Fungal Counterstrategies
TL;DR: Current knowledge about fungal counterstrategies for subverting chitin-triggered plant immunity are summarized, CERK1 functions are outlined, and open questions and future directions in this field are proposed.
Journal ArticleDOI
The Cotton Wall-Associated Kinase GhWAK7A Mediates Responses to Fungal Wilt Pathogens by Complexing with the Chitin Sensory Receptors.
Ping Wang,Ping Wang,Lin Zhou,Pierce Jamieson,Lin Zhang,Zhi-Xue Zhao,Kevin Babilonia,Wenyong Shao,Wenyong Shao,Lizhu Wu,Lizhu Wu,Roma Mustafa,Roma Mustafa,Imran Amin,Alessandra Diomaiuti,Daniela Pontiggia,Simone Ferrari,Yuxia Hou,Ping He,Libo Shan +19 more
TL;DR: Gossypium hirsutum GhWAK7A, a wall-associated kinase, is identified that positively regulates cotton response to both Vd and Fov infections and plays a crucial role in chitin-induced responses.
Journal ArticleDOI
Arabidopsis Transmembrane Receptor-Like Kinases (RLKs): A Bridge between Extracellular Signal and Intracellular Regulatory Machinery
TL;DR: Recent advances are summarized and an updated overview of transmembrane RLKs in Arabidopsis is provided to contribute to a better understanding of the fundamental signaling mechanisms.
Journal ArticleDOI
Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
Cécilia Cheval,Sebastian Samwald,Matthew G. Johnston,Jeroen de Keijzer,Andrew Breakspear,Xiaokun Liu,Annalisa Bellandi,Yasuhiro Kadota,Cyril Zipfel,Cyril Zipfel,Christine Faulkner +10 more
TL;DR: This work characterizes how a cell can produce a localized and specific response in a discrete membrane domain, identifying that there is microdomain specificity in immune signaling to a single elicitor and that cell-to-cell connections are independently controlled.
References
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Journal ArticleDOI
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
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Journal ArticleDOI
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.
TL;DR: The transient gene expression system using Arabidopsis mesophyll protoplasts has proven an important and versatile tool for conducting cell-based experiments using molecular, cellular, biochemical, genetic, genomic and proteomic approaches to analyze the functions of diverse signaling pathways and cellular machineries.
Journal ArticleDOI
A Renaissance of Elicitors: Perception of Microbe-Associated Molecular Patterns and Danger Signals by Pattern-Recognition Receptors
Thomas Boller,Georg Felix +1 more
TL;DR: Current evidence indicates that MAMPs, DAMPs, and effectors are all perceived as danger signals and induce a stereotypic defense response, and the importance of MAMP/PRR signaling for plant immunity is highlighted.
Journal ArticleDOI
A gene expression map of Arabidopsis thaliana development
Markus Schmid,Timothy S. Davison,Stefan R. Henz,Utz Johann Pape,Monika Demar,Martin Vingron,Bernhard Schölkopf,Detlef Weigel,Detlef Weigel,Jan U. Lohmann +9 more
TL;DR: Examining the expression patterns of large gene families, it is found that they are often more similar than would be expected by chance, indicating that many gene families have been co-opted for specific developmental processes.
Journal ArticleDOI
CERK1, a LysM receptor kinase, is essential for chitin elicitor signaling in Arabidopsis
Ayako Miya,Premkumar Albert,Premkumar Albert,Tomonori Shinya,Yoshitake Desaki,Kazuya Ichimura,Ken Shirasu,Yoshihiro Narusaka,Naoto Kawakami,Hanae Kaku,Naoto Shibuya +10 more
TL;DR: CERK1 is a plasma membrane protein containing three LysM motifs in the extracellular domain and an intracellular Ser/Thr kinase domain with autophosphorylation/myelin basic protein kinase activity, suggesting that CERK 1 plays a critical role in fungal MAMP perception in plants.