Lymphocyte subset analyses in healthy adults vaccinated with yellow fever 17DD virus.
Ana Paula de Azevedo dos Santos,Álvaro Luiz Bertho,Daniela Dias,Jaciara Ramos Santos,Rugimar Marcovistz +4 more
TLDR
The levels of neutralizing antibodies of first-time vaccinees were similar to those presented by re-vaccinees at day 30 after vaccination, indicating the success of primary vaccination.Abstract:
In this study the kinetics of humoral and cellular immune responses in first-time vaccinees and re-vaccinees with the yellow fever 17DD vaccine virus was analyzed. Flow cytometric analyses were used to determine percentual values of T and B cells in parallel to the yellow fever neutralizing antibody production. All lymphocyte subsets analyzed were augmented around the 30th post vaccination day, both for first-time vaccinees and re-vaccinees. CD3+ T cells increased from 30.8% (SE +/- 4%) to 61.15% (SE +/- 4.2%), CD4+ T cells from 22.4% (SE +/- 3.6%) to 39.17% (SE +/- 2%) with 43% of these cells corresponding to CD4+CD45RO+ T cells, CD8+ T cells from 15.2% (SE +/- 2.9%) to 27% (SE +/- 3%) with 70% corresponding to CD8+CD45RO+ T cells in first-time vaccinees. In re-vaccinees, the CD3+ T cells increased from 50.7% (SE +/- 3%) to 80% (SE +/- 2.3%), CD4+ T cells from 24.9% (SE +/- 1.4%) to 40% (SE +/- 3%) presenting a percentage of 95% CD4+CD45RO+ T cells, CD8+ T cells from 19.7% (SE +/- 1.8%) to 25% (SE +/- 2%). Among CD8+CD38+ T cells there could be observed an increase from 15 to 41.6% in first-time vaccinees and 20.7 to 62.6% in re-vaccinees. Regarding neutralizing antibodies, the re-vaccinees presented high titers even before re-vaccination. The levels of neutralizing antibodies of first-time vaccinees were similar to those presented by re-vaccinees at day 30 after vaccination, indicating the success of primary vaccination. Our data provide a basis for further studies on immunological behavior of the YF 17DD vaccine.read more
Citations
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Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses
Denis Gaucher,René Therrien,Nadia Kettaf,Bastian R. Angermann,Geneviève Boucher,Abdelali Filali-Mouhim,Janice M. Moser,Riyaz Mehta,Donald Drake,Erika Castro,Rama Akondy,Aline Rinfret,Bader Yassine-Diab,Elias A. Said,Younes Chouikh,Mark J. Cameron,Robert Clum,David J. Kelvin,Roland Somogyi,Larry D. Greller,Robert S. Balderas,Peter Wilkinson,Giuseppe Pantaleo,Jim Tartaglia,Elias K. Haddad,Rafick Pierre Sekaly +25 more
TL;DR: The results clearly show that the immune response to a strong vaccine is preceded by coordinated induction of master transcription factors that lead to the development of a broad, polyfunctional, and persistent immune response that integrates all effector cells of the immune system.
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Yellow fever vaccine
TL;DR: The genus Flavivirus is a member of the family Flaviviridae, a group of small, enveloped, positive-sense, single-strand RNA viruses as mentioned in this paper.
Journal ArticleDOI
Immune activation alters cellular and humoral responses to yellow fever 17D vaccine
Enoch Muyanja,Aloysius Ssemaganda,Pearline Ngauv,Rafael Cubas,Helene Perrin,Divya Srinivasan,Glenda Canderan,Benton Lawson,Jakub Kopycinski,Amanda S. Graham,Dawne K. Rowe,Michaela J. Smith,Denis Gaucher,Sharon Isern,Scott F. Michael,Guido Silvestri,Thomas H. Vanderford,Erika Castro,Giuseppe Pantaleo,Joel Singer,Jill Gillmour,Noah Kiwanuka,Noah Kiwanuka,Annet Nanvubya,C. Schmidt,Josephine Birungi,Josephine H. Cox,Elias K. Haddad,Pontiano Kaleebu,Pontiano Kaleebu,Patricia E. Fast,Rafick Pierre Sekaly,Lydie Trautmann +32 more
TL;DR: It is demonstrated that an activated immune microenvironment prior to vaccination impedes efficacy of the YF-17D vaccine in an African cohort and suggest that vaccine regimens may need to be boosted in African populations to achieve efficient immunity.
Journal ArticleDOI
Dynamics of the Cytotoxic T Cell Response to a Model of Acute Viral Infection
William S DeWitt,Ryan O. Emerson,Paul Lindau,Marissa Vignali,Thomas M. Snyder,Cindy Desmarais,Catherine Sanders,Heidi Utsugi,Edus H. Warren,M. Juliana McElrath,Karen W. Makar,Anna Wald,Harlan Robins +12 more
TL;DR: This is the first study to use high-throughput sequencing of immune cells to characterize the breadth of the antiviral effector cell response and to determine the contribution of unique virus-induced clones to the long-lived memory T cell repertoire.
Journal ArticleDOI
17DD yellow fever vaccine: A double blind, randomized clinical trial of immunogenicity and safety on a dose-response study
Reinaldo de Menezes Martins,Maria de Lourdes de Sousa Maia,Roberto Henrique Guedes Farias,Luiz Antonio Bastos Camacho,Marcos da Silva Freire,Ricardo Galler,Anna M. Y. Yamamura,L.F.C. Almeida,Sheila Maria Barbosa de Lima,Rita Maria Ribeiro Nogueira,Gloria Regina da Silva e Sá,Darcy Akemi Hokama,Ricardo de Carvalho,Ricardo Aguiar Villanova Freire,Edson Pereira Filho,Maria da Luz Fernandes Leal,Akira Homma +16 more
TL;DR: In young healthy adults Bio-Manguinhos/Fiocruz yellow fever vaccine can be used in much lower doses than usual, and is as immunogenic and safe as the current formulation.
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