Journal ArticleDOI
Metal-Organic Framework-Based Enzyme Biocomposites.
Weibin Liang,Peter Wied,Francesco Carraro,Christopher J. Sumby,Bernd Nidetzky,Chia-Kuang Tsung,Paolo Falcaro,Christian J. Doonan +7 more
TLDR
A review of the characterization methodologies used for enzyme/MOF-immobilized enzymes can be found in this article, where the authors discuss enzyme protection via encapsulation, pore infiltration and surface adsorption and summarizes strategies to form multicomponent composites.Abstract:
Because of their efficiency, selectivity, and environmental sustainability, there are significant opportunities for enzymes in chemical synthesis and biotechnology. However, as the three-dimensional active structure of enzymes is predominantly maintained by weaker noncovalent interactions, thermal, pH, and chemical stressors can modify or eliminate activity. Metal-organic frameworks (MOFs), which are extended porous network materials assembled by a bottom-up building block approach from metal-based nodes and organic linkers, can be used to afford protection to enzymes. The self-assembled structures of MOFs can be used to encase an enzyme in a process called encapsulation when the MOF is synthesized in the presence of the biomolecule. Alternatively, enzymes can be infiltrated into mesoporous MOF structures or surface bound via covalent or noncovalent processes. Integration of MOF materials and enzymes in this way affords protection and allows the enzyme to maintain activity in challenge conditions (e.g., denaturing agents, elevated temperature, non-native pH, and organic solvents). In addition to forming simple enzyme/MOF biocomposites, other materials can be introduced to the composites to improve recovery or facilitate advanced applications in sensing and fuel cell technology. This review canvasses enzyme protection via encapsulation, pore infiltration, and surface adsorption and summarizes strategies to form multicomponent composites. Also, given that enzyme/MOF biocomposites straddle materials chemistry and enzymology, this review provides an assessment of the characterization methodologies used for MOF-immobilized enzymes and identifies some key parameters to facilitate development of the field.read more
Citations
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Journal ArticleDOI
Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization.
TL;DR: The pitfalls of using incorrectly designed immobilization protocols are highlighted and why in many cases sub-optimal results are obtained and solutions to overcome these challenges are described.
Journal ArticleDOI
MOF-enabled confinement and related effects for chemical catalyst presentation and utilization.
Jian Liu,Timothy A. Goetjen,Qining Wang,Julia G. Knapp,Megan C. Wasson,Ying-Chao Yang,Zoha H. Syed,Massimiliano Delferro,Justin M. Notestein,Omar K. Farha,Joseph T. Hupp +10 more
TL;DR: This review focuses on how porous, catalyst-containing MOFs capitalize on molecular-scale confinement, containment, isolation, environment modulation, energy delivery, and mobility to accomplish desired chemical transformations with potentially superior selectivity or other efficacy, especially in comparison to catalysts in homogeneous solution environments.
Journal ArticleDOI
Biocatalysis making waves in organic chemistry
TL;DR: A review of the consequences of these waves of development can be found in this article , where the authors reviewed the recent developments of biocatalysis and their impact on chemical synthesis.
Journal ArticleDOI
Recent advances in enzyme immobilization based on novel porous framework materials and its applications in biosensing
TL;DR: In this paper , the authors discuss various strategies of using MOFs/functional MOFs as matrixes to immobilize enzymes, and the latest research progresses of COFs and HOFs in enzyme immobilization are systematically introduced.
Journal ArticleDOI
Target Reprogramming Lysosomes of CD8+ T Cells by a Mineralized Metal-Organic Framework for Cancer Immunotherapy.
Qin Zhao,Zijian Gong,Zhihao Li,Jinyang Wang,Jinglun Zhang,Zifan Zhao,Peng Zhang,Shihang Zheng,Richard J. Miron,Quan Yuan,Yufeng Zhang +10 more
TL;DR: In this article, a lysosome-targeting nanoparticle (LYS-NP) was developed by way of a mineralized metal-organic framework (MOF) coupled with a Lysosomestargeting aptamer (CD63-aptamer) to enhance the antitumor effect of T cells.
References
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Journal ArticleDOI
The Chemistry and Applications of Metal-Organic Frameworks
Hiroyasu Furukawa,Hiroyasu Furukawa,Kyle E. Cordova,Kyle E. Cordova,Michael O'Keeffe,Michael O'Keeffe,Omar M. Yaghi,Omar M. Yaghi,Omar M. Yaghi +8 more
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