Journal ArticleDOI
Mitochondrial D-loop sequences are integrated in the rat nuclear genome.
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TLDR
The mtDNA-like sequences of the nuclear clones hybridize strongly to a number of different BamHI-PstI restriction fragments, suggesting either repeated integration and/or frequent mutational events producing new restriction enzyme sites.About:
This article is published in Journal of Molecular Biology.The article was published on 1991-10-20. It has received 63 citations till now. The article focuses on the topics: Sequence analysis & Restriction fragment.read more
Citations
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Journal ArticleDOI
Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae).
Paul Sunnucks,Dinah F. Hales +1 more
TL;DR: It is concluded that a proportion of the inferred change in the nonmitochondrial sequences occurred before transposition, and that Sitobion aphids (and other species exhibiting mtDNA transposition) may be important for studying the molecular evolution of mtDNA and pseudogenes.
Journal ArticleDOI
Mitochondrial membrane potential.
Ljubava D. Zorova,Vasily A. Popkov,Egor Y. Plotnikov,Denis N. Silachev,Irina B. Pevzner,S. S. Jankauskas,V. A. Babenko,S. D. Zorov,Anastasia V. Balakireva,Magdalena Juhaszova,Steven J. Sollott,Dmitry B. Zorov,Dmitry B. Zorov +12 more
TL;DR: Additional potential mechanisms for which ΔΨm is essential for maintenance of cellular health and viability are proposed and recommendations how to accurately measure ΔΩm in a cell are provided and potential sources of artifacts are discussed.
Journal ArticleDOI
Nuclear integrations: challenges for mitochondrial DNA markers
De-Xing Zhang,Godfrey M. Hewitt +1 more
TL;DR: A better understanding of how the nuclear sequences themselves are interesting, and capable of serving as valuable molecular tools, they can also confound phylogenetic and population genetic studies.
Journal ArticleDOI
Numt, a recent transfer and tandem amplification of mitochondrial DNA to the nuclear genome of the domestic cat.
TL;DR: A recent remarkable transposition of 7.9 kb of a typically 17.0-kb mitochondrial genome to a specific nuclear chromosomal position in the domestic cat is reported, providing an empirical glimpse of historic genomic events that may parallel the accommodation of organelles in eucaryotes.
Journal Article
The retrieval of ancient human DNA sequences
TL;DR: These results show that more experimental work than is often applied is necessary to ensure that DNA sequences amplified from ancient human remains are authentic and quantitation of the numbers of amplifiable molecules is a useful tool to determine the role of contaminating contemporary molecules and PCR errors in amplifications from ancient DNA.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
A comprehensive set of sequence analysis programs for the VAX
TL;DR: A group of programs that will interact with each other has been developed for the Digital Equipment Corporation VAX computer using the VMS operating system.
Journal ArticleDOI
Improved tools for biological sequence comparison.
TL;DR: Three computer programs for comparisons of protein and DNA sequences can be used to search sequence data bases, evaluate similarity scores, and identify periodic structures based on local sequence similarity.
Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.