Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts.
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TLDR
The characterization of human lung fibroblast cDNAs that encode the message for these core proteins and the effect of bacterial phosphatidylinositol-specific phospholipase C suggest that the hydrophobic proteoglycan is membrane-anchored through aospholipid tail.Abstract:
Two mAbs raised against the 64-kD core protein of a membrane heparan sulfate proteoglycan from human lung fibroblasts also recognize a nonhydrophobic proteoglycan which accumulates in the culture medium of the cells. Pulse-chase studies suggest that the hydrophobic cell-associated forms act as precursors for the nonhydrophobic medium-released species. The core proteins of the medium-released proteoglycans are slightly smaller than those of the hydrophobic cell-associated species, but the NH2-terminal amino acid sequences of both forms are identical. The characterization of human lung fibroblast cDNAs that encode the message for these core proteins and the effect of bacterial phosphatidylinositol-specific phospholipase C suggest that the hydrophobic proteoglycan is membrane-anchored through a phospholipid tail. These data identify a novel membrane proteoglycan in human lung fibroblasts and imply that the shedding of this proteoglycan may be related to the presence of the phospholipid anchor.read more
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Journal ArticleDOI
Functions of Cell Surface Heparan Sulfate Proteoglycans
Merton Bernfield,Martin Götte,Pyong Woo Park,Ofer Reizes,Marilyn L. Fitzgerald,John Lincecum,Masahiro Zako +6 more
TL;DR: Current analyses of genetic defects in Drosophila melanogaster, mice, and humans confirm most of these activities in vivo and identify additional processes that involve cell surface heparan sulfate proteoglycans.
Journal ArticleDOI
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
TL;DR: GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.
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Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins
TL;DR: This review summarizes the most recent data on structures and functions of brain proteoglycans and focuses on new physiological concepts for their potential roles in the developing central nervous system.
Journal ArticleDOI
Developmental changes in heparan sulfate expression: in situ detection with mAbs.
TL;DR: The results suggest that heparan sulfate abounds at sites of active morphogenesis and that the expression of this glycosaminoglycan is developmentally regulated.
Journal ArticleDOI
The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule.
Douglas M. Noonan,Anna Fulle,Piera Valente,Silla Cai,Elizabeth A. Horigan,Makoto Sasaki,Yoshihiko Yamada,John R. Hassell +7 more
TL;DR: As the primary structural data agree with the appearance of the molecule in the electron microscope as a series of globules separated by rods, or "beads on a string," the name perlecan is adopted, which suggests multiple interactions with other molecules.
References
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