Journal ArticleDOI
Nanometer fluorescent hybrid silica particle as ultrasensitive and photostable biological labels
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TLDR
These newly developed NFHS particles have been used as a fluorescent marker to label antibodies, using silica immobilization method, for the immunoassay of human alpha-fetoprotein (AFP).Abstract:
Nanometer-sized fluorescent hybrid silica (NFHS) particles were prepared for use as sensitive and photostable fluorescent probes in biological staining and diagnostics. The first step of the synthesis involves the covalent modification of 3-aminopropyltrimethoxysilane with an organic fluorophore, such as fluorescein isothiocyanate, under N2 atmosphere for getting a fluorescent silica precursor. Then the NFHS particles, with a diameter of well below 40 nm, were prepared by controlled hydrolysis of the fluorescent silica precursor with tetramethoxysilane (TMOS) using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. Furthermore, since the fluorophores are covalently bound to the silica network, there is no migration, aggregation and leakage of the fluorophores. In comparison with common single organic fluorophores, these particle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission (blinking). We have used these newly developed NFHS particles as a fluorescent marker to label antibodies, using silica immobilization method, for the immunoassay of human alpha-fetoprotein (AFP). The detection limit of this method was down to 0.05 ng mL(-1) under our current experimental conditions. We think this material would attract much attention and be applied widely in biotechnology.read more
Citations
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Chemistry, Biology, and Medicine of Fluorescent Nanomaterials and Related Systems: New Insights into Biosensing, Bioimaging, Genomics, Diagnostics, and Therapy
Jun Yao,Mei Yang,Yixiang Duan +2 more
TL;DR: This research presents new insights into Biosensing, Bioimaging, Genomics, diagnostics, Diagnostics, and Therapy from the research center of Analytical Instrumentation at Sichuan University.
Journal ArticleDOI
Luminescent nanomaterials for biological labelling
TL;DR: An overview of the recent advances on these luminescent nanomaterials with emphases on their optical characteristics that are crucial in fluorescence microscopy are given, both advantages and limitations in their usage as well as challenges they face, and puts forward the future direction of fluorescent labels in the area of biolabelling.
Journal ArticleDOI
Review: Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles
TL;DR: This review focuses on selected examples to illustrate novel concepts and promising applications of fabricated nanoparticles for biosensing, assay labelling, bioimaging, and in research on a variety of molecular tags in cellular and molecular biology.
Journal ArticleDOI
Nanoparticles in biomolecular detection
Natalia C. Tansil,Zhiqiang Gao +1 more
TL;DR: The use of nanoparticles as labels in biomolecular detection in place of conventional molecular fluorophores has led to improvements in sensitivity, selectivity, and multiplexing capacity as mentioned in this paper.
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Fluorescent dye-doped silica nanoparticles: new tools for bioapplications
TL;DR: Recent developments in the synthesis, biocompatibility, and bioapplications of fluorescent dye-doped silica nanoparticles are summarized.
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Warren C. W. Chan,Shuming Nie +1 more
TL;DR: Highly luminescent semiconductor quantum dots (zinc sulfide-capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection and these nanometer-sized conjugates are water-soluble and biocompatible.
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Synthesis and Characterization of Strongly Luminescing ZnS-Capped CdSe Nanocrystals
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Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science
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Journal ArticleDOI
Multicolor Spectral Karyotyping of Human Chromosomes
Evelin Schröck,S du Manoir,T Veldman,B Schoell,Johannes Wienberg,Malcolm A. Ferguson-Smith,Yi Ning,David H. Ledbetter,Irit Bar-Am,D. Soenksen,Yuval Garini,Thomas Ried +11 more
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