Journal ArticleDOI
Novel role for ALCAM in lymphatic network formation and function
Maria Iolyeva,Sinem Karaman,Ann-Helen Willrodt,Stephanie Weingartner,Benjamin Vigl,Cornelia Halin +5 more
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TLDR
Functional in vitro experiments revealed that ALCAM mediates adhesive interactions, migration, and tube formation in LECs, suggesting a role for A LCAM in lymphatic vessel (LV) stability and in lymphangiogenesis.Abstract:
Adhesion molecules play an important role in vascular biology because they mediate vascular stability, permeability, and leukocyte trafficking to and from tissues. Performing microarray analyses, we have recently identified activated leukocyte cell adhesion molecule (ALCAM) as an inflammation-induced gene in lymphatic endothelial cells (LECs). ALCAM belongs to the immunoglobulin superfamily and engages in homophilic as well as heterophilic interactions. In this study, we found ALCAM to be expressed at the protein level in human and murine lymphatic and blood vascular endothelial cells in vitro and in the vasculature of human and murine tissues in vivo. Functional in vitro experiments revealed that ALCAM mediates adhesive interactions, migration, and tube formation in LECs, suggesting a role for ALCAM in lymphatic vessel (LV) stability and in lymphangiogenesis. Furthermore, ALCAM supported dendritic cell (DC) adhesion to lymphatic endothelium. In agreement with these findings, experiments performed in ALCAM mice detected reduced LEC numbers in various tissues and defects in the formation of an organized LV network. Moreover, DC migration from lung to draining lymph nodes was compromised in ALCAM mice. Collectively, our data reveal a novel role for ALCAM in stabilizing LEC-LEC interactions and in the organization and function of the LV network.read more
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Journal ArticleDOI
A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.
Ryan J. Park,Timothy C. Wang,Dylan Koundakjian,Judd F. Hultquist,Pedro Lamothe-Molina,Pedro Lamothe-Molina,Blandine Monel,Blandine Monel,Kathrin Schumann,Haiyan Yu,Kevin M Krupzcak,Wilfredo F. Garcia-Beltran,Wilfredo F. Garcia-Beltran,Alicja Piechocka-Trocha,Nevan J. Krogan,Nevan J. Krogan,Alexander Marson,David M. Sabatini,Eric S. Lander,Eric S. Lander,Nir Hacohen,Bruce D. Walker +21 more
TL;DR: The findings indicate that HIV infection and replication rely on a limited set of host-dispensable genes and suggest that these pathways can be studied for therapeutic intervention.
Journal ArticleDOI
Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking.
TL;DR: Evidence that LYVE-1 can also bind growth factors and associate with kinase-linked growth factor receptors is evaluated to conclude on how the LYve-1·HA axis may be exploited as a target to either block inflammation or tissue allograft rejection, or potentiate vaccine and drug delivery.
Journal ArticleDOI
Leucocyte Trafficking via the Lymphatic Vasculature- Mechanisms and Consequences
TL;DR: The emerging importance of the leucocyte glycocalyx and its novel interactions with the endothelial receptor LYVE-1, the intricacies of endothelial chemokine secretion and sequestration that direct leucocytes trafficking and the significance of the process for normal immune function and pathology are highlighted.
Journal ArticleDOI
The inflammatory response of lymphatic endothelium
TL;DR: Current knowledge of the inflammatory response of lymphatic endothelium and of inflammation-induced lymphangiogenesis is summarized and the current perspective on the overall functional significance of these processes is discussed.
Journal ArticleDOI
Neutrophils rapidly transit inflamed lymphatic vessel endothelium via integrin-dependent proteolysis and lipoxin-induced junctional retraction.
TL;DR: An unexpectedly intimate collaboration between neutrophils and the lymphatic vessel endothelium is revealed, in which these phagocytic leukocytes act as pathfinders for their own transit during inflammation.
References
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Vascular endothelial growth factor C is required for sprouting of the first lymphatic vessels from embryonic veins.
Marika J. Karkkainen,Paula Haiko,Kirsi Sainio,Juha Partanen,Jussi Taipale,Tatiana V. Petrova,Michael Jeltsch,David A. Jackson,Marja Talikka,Heikki Rauvala,Christer Betsholtz,Kari Alitalo +11 more
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Angiopoietin-2 is required for postnatal angiogenesis and lymphatic patterning, and only the latter role is rescued by Angiopoietin-1.
Nicholas W. Gale,Gavin Thurston,Sean F. Hackett,Roumiana Renard,Quan Wang,Joyce McClain,Cliff Martin,Charles L. Witte,Marlys H. Witte,David A. Jackson,Chitra Suri,Peter A. Campochiaro,Stanley J. Wiegand,George D. Yancopoulos +13 more
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Integrins in angiogenesis and lymphangiogenesis
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TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays.
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Journal ArticleDOI
HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes
Jean-Philippe Girard,Christine Moussion,Christine Moussion,Christine Moussion,Reinhold Förster +4 more
TL;DR: The current understanding of the functions of high endothelial venules, stroma and lymphatics in the entry, positioning and exit of immune cells in lymph nodes during homeostasis are reviewed, and the unexpected role of dendritic cells is highlighted in the control of lymphocyte homing through HEVs.
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