Journal ArticleDOI
Phase Separation in Genome Organization across Evolution
Marina Feric,Tom Misteli +1 more
TLDR
In this paper, the role of phase separation in genome organization across the evolutionary spectrum from bacteria to mammals is discussed, suggesting that molecular interactions among DNA-binding proteins evolved to form a variety of biomolecular condensates with distinct material properties that affect genome organization and function.About:
This article is published in Trends in Cell Biology.The article was published on 2021-03-23. It has received 52 citations till now. The article focuses on the topics: Genome.read more
Citations
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Journal ArticleDOI
The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems
Keren Lasker,Steven Boeynaems,Vinson Lam,Daniel Scholl,Emma Stainton,Adam Briner,Maarten Jacquemyn,Dirk Daelemans,Ashok A. Deniz,Elizabeth Villa,Alex S. Holehouse,Aaron D. Gitler,Lucy Shapiro +12 more
TL;DR: In this paper , the authors show that the material properties of these condensates are determined by a balance between attractive and repulsive forces mediated by a helical oligomerization domain and an expanded disordered region, respectively.
Journal ArticleDOI
Merging Established Mechanisms with New Insights: Condensates, Hubs, and the Regulation of RNA Polymerase II Transcription
TL;DR: In this article, the authors describe the conventional and condensate-dependent models of RNA polymerase II (pol II) and highlight the central role of RNA in these processes, and discuss mutations that disrupt normal partitioning of transcription factors.
Journal ArticleDOI
Merging Established Mechanisms with New Insights: Condensates, Hubs, and the Regulation of RNA Polymerase II Transcription
TL;DR: In this paper , the authors describe the conventional and condensate-dependent models of RNA polymerase II (pol II) and highlight the central role of RNA in these processes, and discuss mutations that disrupt normal partitioning of transcription factors.
Journal ArticleDOI
Mesoscale structure–function relationships in mitochondrial transcriptional condensates
TL;DR: It is found that the core components of the mt-transcription machinery form multiphasic, viscoelastic condensates in vitro, and the rates of condensate-mediated transcription are substantially lower than in solution.
Journal ArticleDOI
Biomolecular condensates: new opportunities for drug discovery and RNA therapeutics.
TL;DR: The potential impact of phase separation on drug discovery and RNA therapeutics, leveraging concrete examples, towards novel clinical opportunities was discussed in this paper . But, the authors did not consider the role of the condensates in the phase separation process.
References
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Journal ArticleDOI
Biomolecular condensates: organizers of cellular biochemistry
TL;DR: This work has shown that liquid–liquid phase separation driven by multivalent macromolecular interactions is an important organizing principle for biomolecular condensates and has proposed a physical framework for this organizing principle.
Journal ArticleDOI
Design and self-assembly of two-dimensional DNA crystals
TL;DR: The design and observation of two-dimensional crystalline forms of DNA that self-assemble from synthetic DNA double-crossover molecules that create specific periodic patterns on the nanometre scale are reported.
Journal ArticleDOI
Liquid phase condensation in cell physiology and disease.
TL;DR: The findings together suggest that several membrane-less organelles have been shown to exhibit a concentration threshold for assembly, a hallmark of phase separation, and represent liquid-phase condensates, which form via a biologically regulated (liquid-liquid) phase separation process.
Journal ArticleDOI
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
Clifford P. Brangwynne,Christian R. Eckmann,David S. Courson,Agata Rybarska,Carsten Hoege,Jöbin Gharakhani,Jöbin Gharakhani,Frank Jülicher,Frank Jülicher,Anthony A. Hyman,Anthony A. Hyman +10 more
TL;DR: It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell.
Journal ArticleDOI
Liquid-liquid phase separation in biology.
TL;DR: The basic physical concepts necessary to understand the consequences of liquid-like states for biological functions are discussed.
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Benjamin R. Sabari,Alessandra Dall’Agnese,Ann Boija,Isaac A. Klein,Isaac A. Klein,Eliot L. Coffey,Krishna Shrinivas,Brian J. Abraham,Nancy M. Hannett,Alicia V. Zamudio,John C. Manteiga,Charles H. Li,Yang Eric Guo,Daniel S. Day,Jurian Schuijers,Eliza Vasile,Sohail Malik,Denes Hnisz,Tong Ihn Lee,Ibrahim I Cisse,Robert G. Roeder,Phillip A. Sharp,Arup K. Chakraborty,Richard A. Young +23 more