Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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Journal ArticleDOI
A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
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Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
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Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
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Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
Journal ArticleDOI
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Photoacoustic flow measurements based on wash-in analysis of gold nanorods
TL;DR: In this study, photoacoustic flow measurement methods based on wash-in analysis are presented, which use the rod-to-sphere shape transformations of gold nanorods induced by pulsed-laser irradiation to fit the time-intensity curves by exponential models.
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Single-wavelength functional photoacoustic microscopy in biological tissue
TL;DR: The oxygen saturation was quantified in vivo with single-wavelength photoac acoustic microscopy, eliminating the need for multiwavelength measurements and improves the portability and cost-effectiveness of functional or molecular photoacoustic microscopy.
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Label-free imaging of zebrafish larvae in vivo by photoacoustic microscopy
TL;DR: It is demonstrated that this method has the potential to be a powerful non-invasive imaging method for studying various small animal models, including zebrafish larvae, Caenorhabditis elegans, frogs and drosophila larvae.
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In vivo Photoacoustic Tomography of Total Blood Flow and Potential Imaging of Cancer Angiogenesis and Hypermetabolism
TL;DR: A method to compute the total flow speed and Doppler angle by combining the axial and transverse flow measurements is proposed and believes that the proposed method has the potential to be used for cancer angiogenesis and hypermetabolism imaging.
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Functional photoacoustic microscopy of diabetic vasculature
TL;DR: To produce an animal model for Type 1 diabetes, streptozotocin (STZ) was used, which is particularly toxic to the insulin-producing beta cells of the pancreas in mammals, to image diabetes-induced damage to the microvasculature.