Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
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TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
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Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
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Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
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Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
Journal ArticleDOI
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Nanoparticles for photoacoustic imaging
TL;DR: The design and application of each nanoparticle-based contrast agent in relation to the field of PAI are detailed and particular focus is given to nanoparticles whose absorption mechanism is based on surface plasmon resonance.
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Noninvasive photoacoustic angiography of animal brains in vivo with near-infrared light and an optical contrast agent
TL;DR: A photoacoustic angiograph of a rat brain is acquired that matches the anatomical photograph well and exhibits high spatial resolution and a much-reduced background and demonstrates the potential for dynamic and molecular biomedical imaging.
Book
Basics of Biomedical Ultrasound for Engineers
TL;DR: In this article, the authors present a general overview of the application of ultrasound in medicine, focusing on the applicability of this technology in the field of medical imaging. But they do not discuss any specific applications.
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In vivo measurements of the wavelength dependence of tissue-scattering coefficients between 760 and 900 nm measured with time-resolved spectroscopy
TL;DR: In vivo values for the optical transport coefficients for the adult head are substantially lower than previously reported values for adult human cerebral matter and pig skull cortical bone measured in vitro.
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Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo
TL;DR: In vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) is developed that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments and can resolve subcellular organelles.