Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
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TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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Proceedings ArticleDOI
High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae
TL;DR: In this article, a high frequency, high numerical aperture spherically focused ultrasound transducer, with a wide bandwidth of 25-125 MHz, was used for optoacoutic mesoscopy where the excitation was performed using a diode laser capable of >500 μJ/pulse, 1.8ns pulse width and 1.4 kHz pulse repetition rate.
Journal ArticleDOI
Listen to the chemical and histological information in biological tissue
TL;DR: Photoacoustic imaging inherits the high spatial resolution of ultrasonography in imaging deep tissue and the high sensitivity of optical imaging in evaluating tissue chemical and physiological information.
Journal ArticleDOI
Super-Resolution Photoacoustic Microscopy via Modified Phase Compounding
TL;DR: In this article , a modified phase-shifting method is used to generate the second harmonic of the fringes and double the spectral shift, which can increase the lateral resolution by five folds.
Book ChapterDOI
Photoacoustic tomography and its applications
TL;DR: In this paper , the basic features of signal generation and detection during photo-acoustic imaging are presented, and the emerging noninvasive approaches for detection of early and metastatic cancer, PA image guiding therapy, and assessment of therapeutic response are discussed.
Proceedings ArticleDOI
All-optical forward-view photoacoustic endoscopy
TL;DR: In this paper, the authors presented a miniature all-optical probe that enables forward-view photoacoustic endoscopy without using a scanner, which utilizes an imaging fiber bundle and a gradient-index (GRIN) objective lens for delivery, focusing, and scanning of excitation laser pulses.
References
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Journal ArticleDOI
Hallmarks of cancer: the next generation.
TL;DR: Recognition of the widespread applicability of these concepts will increasingly affect the development of new means to treat human cancer.
Journal ArticleDOI
The hallmarks of cancer.
TL;DR: This work has been supported by the Department of the Army and the National Institutes of Health, and the author acknowledges the support and encouragement of the National Cancer Institute.
Journal ArticleDOI
Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs
Lihong V. Wang,Song Hu +1 more
TL;DR: A review of the state of the art of photoacoustic tomography for both biological and clinical studies can be found in this paper, where the authors discuss the current state-of-the-art and discuss future prospects.
Journal ArticleDOI
Nanoparticle and targeted systems for cancer therapy.
TL;DR: This review explores recent work directed towards more targeted treatment of cancer, whether through more specific anti-cancer agents or through methods of delivery, including delivery by avoiding the reticuloendothelial system, utilizing the enhanced permeability and retention effect and tumor-specific targeting.
Journal ArticleDOI
Nanoparticles in Medicine: Therapeutic Applications and Developments
TL;DR: Nanomaterials have unique physicochemical properties, such as ultra small size, large surface area to mass ratio, and high reactivity, which are different from bulk materials of the same composition, which can be used to overcome some of the limitations found in traditional therapeutic and diagnostic agents.