Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
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TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
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Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
Journal ArticleDOI
Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
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Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
Journal ArticleDOI
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Noninvasive, in vivo imaging of blood-oxygenation dynamics within the mouse brain using photoacoustic microscopy
TL;DR: The ability of PAM to track blood oxygenation in the mouse brain is confirmed, a critical aspect of imaging brain activity through the hemodynamic response, in response to controlled hypoxic and hyperoxic challenges.
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Intravascular Photoacoustic Imaging
Bo Wang,Jimmy L. Su,Andrei B. Karpiouk,Konstantin V Sokolov,Richard W. Smalling,Stanislav Emelianov +5 more
TL;DR: Assessment of the composition of atherosclerotic plaques, imaging of macrophages within the plaque, and molecular imaging of biomarkers associated with formation and development of plaques are presented.
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Quantitative determination of chromophore concentrations from 2D photoacoustic images using a nonlinear model-based inversion scheme
TL;DR: A model-based inversion scheme was used to determine absolute chromophore concentrations from multiwavelength photoacoustic images, which incorporated a forward model, which predicted 2D images of the initial pressure distribution as a function of the spatial distribution of the chromophor concentrations.
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Detection of ultrawide-band ultrasound pulses in optoacoustic tomography
TL;DR: The detector design considerations essential for obtaining high-quality optoacoustic images are presented and physical principles of an array of ultrawide-band ultrasonic transducers used in LOIS designed for imaging tumors in the depth of tissue are described.
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Curved array photoacoustic tomographic system for small animal imaging
John Gamelin,Andres Aguirre,Anastasios Maurudis,Fei Huang,Diego Castillo,Lihong V. Wang,Quing Zhu +6 more
TL;DR: For the first time, according to the knowledge, resolution of sub-200-microm vessels with an overlying turbid medium of greater than 2 cm depth is demonstrated using only intrinsic biological contrast.