Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
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TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
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Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
Journal ArticleDOI
Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
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Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
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IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Prospects of photoacoustic tomography
TL;DR: The prospects of photoacoustic tomography are envisaged in the following aspects:photoacoustic microscopy of optical absorption emerging as a mainstream technology, melanoma detection using photoac acoustic microscopy, and multiscale photoacoust imaging in vivo with common signal origins.
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Multifunctional nanoparticles as coupled contrast agents
TL;DR: In this paper, the authors demonstrate iron oxide and gold-coupled core-shell nanoparticles (NPs) with well-defined structural characteristics (e.g., size, shell thickness, and coreshell separation) and physical properties (i.e., electronic, magnetic, optical, thermal and acoustic).
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In vivo molecular photoacoustic tomography of melanomas targeted by bioconjugated gold nanocages.
Chulhong Kim,Eun Chul Cho,Jingyi Chen,Kwang Hyun Song,Leslie Au,Christopher P. Favazza,Qiang Zhang,Claire M. Cobley,Feng Gao,Younan Xia,Lihong V. Wang +10 more
TL;DR: High-resolution photoacoustic tomography (PAT) with extraordinarily optical absorbing gold nanocages (AuNCs) can serve as a novel contrast agent for in vivo molecular PAT of melanomas with both exquisite sensitivity and high specificity.
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Photoacoustic tomography of a nanoshell contrast agent in the in vivo rat brain
Yiwen Wang,Xueyi Xie,Xueding Wang,Geng Ku,Kelly L. Gill,D. Patrick O'Neal,George Stoica,Lihong V. Wang +7 more
TL;DR: This study demonstrates the feasibility of using nanoshells in vivo as a new contrast-enhancing agent for photoacoustic tomography and presents a gradual enhancement of the optical absorption in the brain vessels by up to 63%.
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In vivo dark-field reflection-mode photoacoustic microscopy.
TL;DR: Reflection-mode photoacoustic microscopy with dark-field laser pulse illumination and high-numerical-aperture ultrasonic detection is designed and implemented in noninvasively imaged blood vessels in the skin in vivo, capable of imaging optical-absorption contrast as deep as 3 mm in biological tissue.