Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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Journal ArticleDOI
A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
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Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
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Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
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Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
Journal ArticleDOI
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Lifetime-based photoacoustic oxygen sensing in vivo
TL;DR: A lifetime-based photoacoustic technique for the measurement of oxygen in vivo, using an oxygen sensitive dye, enabling real time quantification of blood oxygenation, is demonstrated for the first time.
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Noninvasive photoacoustic imaging of the thoracic cavity and the kidney in small and large animals
Kwang Hyun Song,Lihong V. Wang +1 more
TL;DR: This study shows the deep internal organ imaging capability of the deeply penetrating reflection-mode photoacoustic imaging system in animals, which can potentially be used to study tumors in internal organs, and be adapted to clinical diagnosis.
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Polymer microring resonators for high-sensitivity and wideband photoacoustic imaging
TL;DR: A simulation of photoacoustic tomography of beads shows that objects with their boundaries characteristic of high spatial frequencies and the inner structure primarily of low spatial frequency components can be faithfully reconstructed using such a detector.
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Label-free photoacoustic microscopy of myocardial sheet architecture
TL;DR: High-resolution label-free photoacoustic microscopy (PAM) of the myocardial sheet architecture is implemented and has the potential for the functional imaging of sheet architecture in ex vivo perfused and viable hearts.
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Photoacoustic microscopy of blood pulse wave
TL;DR: Electrocardiogram-synchronized, photoacoustic microscopy for noninvasive quantification of the blood pulse wave velocity in the peripheral vessels of living mice shows a linear correlation between the PWV and the vessel diameter which agrees with known physiology.