Photoacoustic microscopy: Photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TLDR
Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.Abstract:
Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.read more
Citations
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Journal ArticleDOI
A practical guide to photoacoustic tomography in the life sciences
Lihong V. Wang,Junjie Yao +1 more
TL;DR: The fundamentals of photoacoustic tomography are reviewed and practical guidelines for matching PAT systems with research needs are provided, and the most promising biomedical applications of PAT are summarized.
Journal ArticleDOI
Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
Journal ArticleDOI
Photoacoustic clinical imaging
Idan Steinberg,David M. Huland,Ophir Vermesh,Hadas Frostig,Willemieke S. Tummers,Sanjiv S. Gambhir +5 more
TL;DR: The various clinical and pre-clinical literature is surveyed and the potential applications and hurdles that still need to be overcome are discussed.
Journal ArticleDOI
Sensitivity of photoacoustic microscopy
Junjie Yao,Lihong V. Wang +1 more
TL;DR: The detection sensitivity of PAM is discussed, the detection efficiency of different PAM designs are compared, and the imaging performance of various endogenous and exogenous contrast agents is summarized.
Journal ArticleDOI
IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control
Arun Thitaikumar,Jonathan Ophir +1 more
TL;DR: The IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control (TUFFC) now accepts color figures online with corresponding grayscale figures in print without additional charges if authors follow the multimedia manuscript submission procedure in the “Information for Contributors”.
References
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Book
Biomedical Optics: Principles and Imaging
Lihong V. Wang,Hsin‐I Wu +1 more
TL;DR: In this paper, the authors present a method for computing the probability density function of a Gaussian beam in a single-spectral image with respect to the Rayleigh theory of light-matter interaction.
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Universal back-projection algorithm for photoacoustic computed tomography.
Minghua Xu,Lihong V. Wang +1 more
TL;DR: In this article, a universal back-projection algorithm for three-dimensional photoacoustic computed tomography is presented for three types of imaging geometries: planar, spherical, and cylindrical surfaces.
Journal ArticleDOI
Nuclear structure in cancer cells.
TL;DR: Advances in understanding nuclear structure have revealed insights into the process of malignant transformation and provide a basis for the development of new diagnostic tools and therapeutics.
Journal ArticleDOI
Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries.
TL;DR: In vivo imaging of the microvasculature including single capillaries in mice using optical-resolution photoacoustic microscopy (OR-PAM) developed in the laboratory is presented.
Book
Foundations of Biomedical Ultrasound
TL;DR: The most comprehensive book on the subject, Foundations of Biomedical Ultrasound is an indispensable reference for any medical professional working with ultrasound imaging, and a comprehensive introduction to the subject for students.