Journal ArticleDOI
Real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer.
Ramadass Balamurugan,Ethendhar Rajendiran,Sarah George,G Vijay Samuel,Balakrishnan S. Ramakrishna +4 more
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TLDR
The present study quantitated Eubacterium rectale, Faecalibacterium prausnitzii, Desulfovibrio (sulfate‐reducing bacteria), and Enterococcus faecalis (that produces extracellular superoxide) in the feces of patients with colorectal cancer.Abstract:
Background and Aim: Bacterial metabolites produced in the bowel are potentially related to the genesis of colorectal cancer. Butyrate is protective against cancer, whereas hydrogen sulfide and oxygen free radicals can be toxic to the epithelium. The present study was designed to quantitate Eubacterium rectale, Faecalibacterium prausnitzii (both butyrate-producing bacteria), Desulfovibrio (sulfate-reducing bacteria), and Enterococcus faecalis (that produces extracellular superoxide) in the feces of patients with colorectal cancer. Methods: DNA was extracted from feces of 20 patients with colorectal cancer, nine patients with upper gastrointestinal cancer and 17 healthy volunteers. Real-time polymerase chain reaction using primers aimed at 16S rDNA was used to quantitate the above bacterial species or genus, and this was expressed relative to amplification of universal sequences conserved among all bacteria. Results: Levels of E. rectale and F. prausnitzii were decreased approximately fourfold (P = 0.0088 and 0.0028, respectively) in colorectal cancer patients compared to healthy control volunteers. Levels of Desulfovibrio were not significantly different between the three groups. E. faecalis populations were significantly higher in colorectal cancer patients compared to healthy volunteers (P = 0.0294). Conclusions: Butyrate producers were decreased and E. faecalis increased in the feces of colon cancer patients. These shifts in the colonic bacterial population could potentially lead to epithelial cell damage and increased turnover and may be a factor leading to colon cancer.read more
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Journal ArticleDOI
Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers
Tingting Wang,Guoxiang Cai,Yunping Qiu,Na Fei,Menghui Zhang,Xiaoyan Pang,Wei Jia,Sanjun Cai,Liping Zhao +8 more
TL;DR: Reduction of butyrate-producing bacteria in the gut microbiota of CRC patients and increase of opportunistic pathogens may constitute a major structural imbalance of gut microbiota in CRC patients.
Journal ArticleDOI
The Warburg Effect Dictates the Mechanism of Butyrate-Mediated Histone Acetylation and Cell Proliferation
TL;DR: It is demonstrated that metabolic transformation plays an important role in the development of cancer cells and that butyrate stimulated the proliferation of normal colonocytes and cancerous colonocytes when the Warburg effect was prevented from occurring.
Journal ArticleDOI
Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii influence the production of mucus glycans and the development of goblet cells in the colonic epithelium of a gnotobiotic model rodent.
Laura Wrzosek,Sylvie Miquel,Marie-Louise Noordine,Stephan Bouet,Stephan Bouet,Marie Joncquel Chevalier-Curt,Marie Joncquel Chevalier-Curt,Véronique Robert,Catherine Philippe,Chantal Bridonneau,Claire Cherbuy,Catherine Robbe-Masselot,Catherine Robbe-Masselot,Philippe Langella,Muriel Thomas +14 more
TL;DR: The importance of the balance between two main commensal bacteria in maintaining colonic epithelial homeostasis via their respective effects on mucus is revealed via the influence of B. thetaiotaomicron and F. prausnitzii.
Journal ArticleDOI
Gut microbiota imbalance and colorectal cancer.
Johan Gagnière,Jennifer Raisch,Julie Veziant,Nicolas Barnich,Richard Bonnet,Emmanuel Buc,Marie-Agnès Bringer,Denis Pezet,Mathilde Bonnet +8 more
TL;DR: The possible links between the bacterial microbiota and colorectal carcinogenesis are discussed, focusing on dysbiosis and the potential pro-carcinogenic properties of bacteria, such as genotoxicity and other virulence factors, inflammation, host defenses modulation, bacterial-derived metabolism, oxidative stress and anti-oxidative defenses modulation.
Journal ArticleDOI
Faecalibacterium prausnitzii : from microbiology to diagnostics and prognostics
TL;DR: This species may be a useful potential biomarker to assist in ulcerative colitis and Crohn’s disease discrimination and its application as a biomarker for diagnostics and prognostics of gut diseases are discussed.
References
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Bert Vogelstein,Eric R. Fearon,Stanley R. Hamilton,Scott E. Kern,Ann C. Preisinger,Mark Leppert,A M Smits,Johannes L. Bos +7 more
TL;DR: It is found that ras-gene mutations occurred in 58 percent of adenomas larger than 1 cm and in 47 percent of carcinomas, which are consistent with a model of colorectal tumorigenesis in which the steps required for the development of cancer often involve the mutational activation of an oncogene coupled with the loss of several genes that normally suppress tumors.
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TL;DR: The design and evaluation of a set of universal primers and probe for the amplification of 16S rDNA from the Domain Bacteria to estimate total bacterial load by real-time PCR is reported, and the number of anaerobic bacteria estimated by the universal probe and primers set in carious dentine was 40-fold greater than the totalacterial load detected by culture methods, demonstrating the utility of real- time PCR in the analysis of this environment.
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