Recommendation on Design, Execution, and Reporting of Animal Atherosclerosis Studies: A Scientific Statement From the American Heart Association
Alan Daugherty,Alan R. Tall,Mat J.A.P. Daemen,Erling Falk,Edward A. Fisher,Guillermo García-Cardeña,Aldons J. Lusis,A. Phillip Owens,Michael E. Rosenfeld,Renu Virmani +9 more
TLDR
Recommendations include the following: (1) animal model selection, with commentary on the fidelity of mimicking facets of the human disease; (2) experimental design and its impact on the interpretation of data; and (3) standard methods to enhance accuracy of measurements and characterization of atherosclerotic lesions.Abstract:
Animal studies are a foundation for defining mechanisms of atherosclerosis and potential targets of drugs to prevent lesion development or reverse the disease. In the current literature, it is common to see contradictions of outcomes in animal studies from different research groups, leading to the paucity of extrapolations of experimental findings into understanding the human disease. The purpose of this statement is to provide guidelines for development and execution of experimental design and interpretation in animal studies. Recommendations include the following: (1) animal model selection, with commentary on the fidelity of mimicking facets of the human disease; (2) experimental design and its impact on the interpretation of data; and (3) standard methods to enhance accuracy of measurements and characterization of atherosclerotic lesions.read more
Citations
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Abdominal aortic aneurysms.
Natzi Sakalihasan,Jean-Baptiste Michel,Athanasios Katsargyris,Helena Kuivaniemi,Jean-Olivier Defraigne,Alain Nchimi,Alain Nchimi,Janet T. Powell,Koichi Yoshimura,Koichi Yoshimura,Rebecka Hultgren,Rebecka Hultgren +11 more
TL;DR: Elective repair of AAA with open surgery or endovascular aortic repair (EVAR) should be considered to prevent AAA rupture, although the morbidity and mortality associated with both techniques remain non-negligible.
Journal ArticleDOI
Treatment of atherosclerosis by macrophage-biomimetic nanoparticles via targeted pharmacotherapy and sequestration of proinflammatory cytokines
Cheng Gao,Qiaoxian Huang,Conghui Liu,Cheryl H. T. Kwong,Ludan Yue,Jian-Bo Wan,Simon Ming-Yuen Lee,Ruibing Wang +7 more
TL;DR: Comparison to macrophage internalized with ROS-responsive NPs, as a live-cell based drug delivery system for treatment of atherosclerosis, suggests that cell membrane coated drug delivery approach is likely more suitable for dealing with an inflammatory disease than the live- cell approach.
Journal ArticleDOI
Consideration of Sex Differences in Design and Reporting of Experimental Arterial Pathology Studies—Statement From ATVB Council
Peggy Robinet,Dianna M. Milewicz,Lisa A. Cassis,Nicholas J. Leeper,Hong Lu,Jonathan D. Smith +5 more
TL;DR: In this article, the authors suggest guidelines for designing and reporting sex as a biological variable in animal models of atherosclerosis, thoracic and abdominal aortic aneurysms, and peripheral arterial disease.
Journal ArticleDOI
Age-Associated Mitochondrial Dysfunction Accelerates Atherogenesis
Daniel J. Tyrrell,Muriel G. Blin,Jianrui Song,Sherri C. Wood,Min Zhang,Daniel A. Beard,Daniel R. Goldstein +6 more
TL;DR: In this paper, the authors show that aging is one of the strongest risk factors for atherosclerosis and that it is not known whether aging increases the risk of coronary heart disease independently of chronic hyperlipidemia.
Journal ArticleDOI
Role of p38 MAPK in Atherosclerosis and Aortic Valve Sclerosis
Anna Reustle,Michael Torzewski +1 more
TL;DR: Experimental findings relating to p38 MAPK in atherosclerosis and aortic valve sclerosis are summarized and potential functions of p38MAPK in the diseases are discussed with the aim of clarifying its eligibility as a pharmacological target.
References
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RNA-Seq: a revolutionary tool for transcriptomics
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TL;DR: This study has demonstrated that careful use of a shared control group represents a safe and effective approach to GWA analyses of multiple disease phenotypes; generated a genome-wide genotype database for future studies of common diseases in the British population; and shown that, provided individuals with non-European ancestry are excluded, the extent of population stratification in theBritish population is generally modest.
Journal ArticleDOI
Prediction of Coronary Heart Disease Using Risk Factor Categories
Peter W.F. Wilson,Ralph B. D'Agostino,Daniel Levy,Albert M. Belanger,Halit Silbershatz,William B. Kannel +5 more
TL;DR: A simple coronary disease prediction algorithm was developed using categorical variables, which allows physicians to predict multivariate CHD risk in patients without overt CHD.
Journal ArticleDOI
Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal Research
TL;DR: Most of the papers surveyed did not report using randomisation or blinding to reduce bias in animal selection and outcome assessment, consistent with reviews of many research areas, including clinical studies, published in recent years.
Journal ArticleDOI
Recommendations for Blood Pressure Measurement in Humans and Experimental Animals Part 1: Blood Pressure Measurement in Humans: A Statement for Professionals From the Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research
Thomas G. Pickering,John E. Hall,Lawrence J. Appel,Bonita Falkner,John W. Graves,Martha N. Hill,Daniel W. Jones,Theodore W. Kurtz,Sheldon G. Sheps,Edward J. Roccella +9 more
TL;DR: It is increasingly recognized that office measurements correlate poorly with blood pressure measured in other settings, and that they can be supplemented by self-measured readings taken with validated devices at home, which gives a better prediction of risk than office measurements and is useful for diagnosing white-coat hypertension.
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