Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression
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This article is published in Nucleic Acids Research.The article was published on 1994-01-01 and is currently open access. It has received 1011 citations till now. The article focuses on the topics: Heterologous expression & Saccharomyces cerevisiae.read more
Citations
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Genomic expression programs in the response of yeast cells to environmental changes.
Audrey P. Gasch,Paul T. Spellman,Camilla M. Kao,Orna Carmel-Harel,Michael B. Eisen,Gisela Storz,David Botstein,Patrick O. Brown +7 more
TL;DR: Analysis of genomic expression patterns in the yeast Saccharomyces cerevisiae implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators.
Journal ArticleDOI
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
Carsten Janke,Maria M. Magiera,Nicole Rathfelder,Christof Taxis,Simone Reber,Hiromi Maekawa,Alexandra C. Moreno-Borchart,Georg Doenges,Etienne Schwob,Elmar Schiebel,Michael Knop,Michael Knop +11 more
TL;DR: Using the provided cassettes for N‐ and C‐terminal gene tagging or for deletion of any given gene, a set of only four primers is required, which makes this method very cost‐effective and reproducible.
Journal ArticleDOI
A New Efficient Gene Disruption Cassette for Repeated Use in Budding Yeast
TL;DR: A loxP-kanMX-loxP gene disruption cassette is developed, which combines the advantages of the heterologous kanr marker with those from the Cre- lox P recombination system, and will be of great advantage for the functional analysis of gene families.
Journal ArticleDOI
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
TL;DR: The use of type II bacterial CRISPR-Cas system in Saccharomyces cerevisiae for genome engineering provides foundations for a simple and powerful genome engineering tool for site-specific mutagenesis and allelic replacement in yeast.
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A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
TL;DR: It is concluded that exposure of a three amino acid motif (RKR) can explain how assembly of an ion channel complex is coupled to intracellular trafficking.
References
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A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Robert S. Sikorski,Philip Hieter +1 more
TL;DR: A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
Journal Article
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
Journal ArticleDOI
A novel genetic system to detect protein-protein interactions.
Stanley Fields,Ok-kyu Song +1 more
TL;DR: A novel genetic system to study protein-protein interactions between two proteins by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae, which may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.
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Multifunctional yeast high-copy-number shuttle vectors
TL;DR: A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces cerevisiae 2 mu endogenous plasmid has been constructed, providing high-copy-number counterparts to the current pRS vectors.
Journal ArticleDOI
DNA sequence required for efficient transcription termination in yeast.
Kenneth S. Zaret,Fred Sherman +1 more
TL;DR: A consensus sequence is uncovered between the region deleted in cyc1-512 and the 3' nontranslated regions of some but not all yeast genes, and the possible role of this sequence in transcription termination is discussed.