Regulation of mRNA cap methylation
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TLDR
The present review discusses how the 7-methylguanosine cap is synthesized by cellular enzymes, the impact that the7- methylguanoine cap has on biological processes, and how the mRNA cap methylation reaction is regulated.Abstract:
The 7-methylguanosine cap added to the 5' end of mRNA is essential for efficient gene expression and cell viability. Methylation of the guanosine cap is necessary for the translation of most cellular mRNAs in all eukaryotic organisms in which it has been investigated. In some experimental systems, cap methylation has also been demonstrated to promote transcription, splicing, polyadenylation and nuclear export of mRNA. The present review discusses how the 7-methylguanosine cap is synthesized by cellular enzymes, the impact that the 7-methylguanosine cap has on biological processes, and how the mRNA cap methylation reaction is regulated.read more
Citations
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mRNA capping: biological functions and applications
TL;DR: This review will summarize the current knowledge of the biological roles of mRNA caps in eukaryotic cells, different means that viruses and their host cells use to cap their RNA and the application of these capping machineries to synthesize functional mRNA.
Journal ArticleDOI
RNA nucleotide methylation
Yuri Motorin,Mark Helm +1 more
TL;DR: This review outlines the different functions of methyl groups in RNA, including biophysical, biochemical and metabolic stabilization of RNA, quality control, resistance to antibiotics, mRNA reading frame maintenance, deciphering of normal and altered genetic code, selenocysteine incorporation, tRNA aminoacylation, ribotoxins, splicing, intracellular trafficking, immune response, and others.
Journal ArticleDOI
The Pivotal Regulatory Landscape of RNA Modifications
Sheng Li,Christopher E. Mason +1 more
TL;DR: Both labile and permanent modifications, from simple methylation to complex transcript alteration (RNA editing and intron retention) are examined; the models for their processing are detailed; and remaining questions in the field of the epitranscriptome are highlighted.
Journal ArticleDOI
Cap-specific terminalN6-methylation of RNA by an RNA polymerase II-associated methyltransferase.
S. Akichika,Seiichi Hirano,Y. Shichino,Toshiharu Suzuki,Hiroshi Nishimasu,Ryuichiro Ishitani,A. Sugita,Y. Hirose,Shintaro Iwasaki,Osamu Nureki +9 more
TL;DR: A cap-specific m6A writer N6,2′-O-dimethyladenosine (m6Am) is present at the transcription start nucleotide of capped mRNAs in vertebrates and RNA-MS analysis revealed that m6Am modification in human m RNAs is more abundant than previously estimated.
Journal ArticleDOI
Transcriptome-wide Mapping of Internal N7-Methylguanosine Methylome in Mammalian mRNA.
Li-Sheng Zhang,Chang Liu,Chang Liu,Honghui Ma,Qing Dai,Qing Dai,Hui-Lung Sun,Hui-Lung Sun,Guan-Zheng Luo,Zijie Scott Zhang,Zijie Scott Zhang,Linda Zhang,Linda Zhang,Lulu Hu,Lulu Hu,Xueyang Dong,Chuan He,Chuan He +17 more
TL;DR: This work developed a chemical-assisted sequencing approach that selectively converts internal m7G sites into abasic sites, inducing misincorporation at these sites during reverse transcription and enabled transcriptome-wide mapping of m7g in human tRNA and mRNA, revealing distribution features of the internal m 7G methylome in human cells.
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