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Review of empirical and emerging breeding methods and tools for yam (Dioscorea spp.) improvement: Status and prospects

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This article is published in Plant Breeding.The article was published on 2020-06-01 and is currently open access. It has received 61 citations till now. The article focuses on the topics: Molecular breeding.

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An underutilized orphan tuber crop—Chinese yam : a review

TL;DR: This article summarizes the sparse research landscape and evaluates the nutritional and medical applications of Chinese yam to encourage the adoption of this orphan crop as a novel functional food.
Journal ArticleDOI

Floral Biology and Pollination Efficiency in Yam (Dioscorea spp.)

TL;DR: There are many limitations in basic and applied knowledge of yam flower biology and pollination, and methods of improving pollination efficiency in yam breeding programs are reviewed.
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Chromosome evolution and the genetic basis of agronomically important traits in greater yam

TL;DR: In this article , the authors presented a highly contiguous chromosome-scale genome assembly of Dioscorea alata combined with a dense genetic map derived from African breeding populations, and found quantitative trait loci for resistance to anthracnose, a damaging fungal pathogen of yam.
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Genome-wide association studies for sex determination and cross-compatibility in water yam (Dioscorea alata L.)

TL;DR: In this paper, the authors used the genome-wide association study (GWAS) approach for identifying genomic regions linked to sex and cross-compatibility in water yam (Dioscorea alata L.).
References
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Journal ArticleDOI

A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species

TL;DR: A procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs) is reported, which is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches.
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Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

TL;DR: In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.
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High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

TL;DR: With its exceptional precision, SpCas9-HF1 provides an alternative to wild-type Sp Cas9 for research and therapeutic applications and suggests a general strategy for optimizing genome-wide specificities of other CRISPR-RNA-guided nucleases.
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Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

TL;DR: Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.
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Potential of metabolomics as a functional genomics tool

TL;DR: This work proposes community-based efforts to define common criteria and to initiate concerted actions directed towards the release of standard reference materials, construction of consolidated metabolite libraries, and development of metabolite-specific data-management systems.
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