A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species
Robert J. Elshire,Jeffrey C. Glaubitz,Qi-ying Sun,Jesse Poland,Ken Kawamoto,Edward S. Buckler,Edward S. Buckler,Sharon E. Mitchell +7 more
TLDR
A procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs) is reported, which is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches.Abstract:
Advances in next generation technologies have driven the costs of DNA sequencing down to the point that genotyping-by-sequencing (GBS) is now feasible for high diversity, large genome species. Here, we report a procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs). This approach is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches. By using methylation-sensitive REs, repetitive regions of genomes can be avoided and lower copy regions targeted with two to three fold higher efficiency. This tremendously simplifies computationally challenging alignment problems in species with high levels of genetic diversity. The GBS procedure is demonstrated with maize (IBM) and barley (Oregon Wolfe Barley) recombinant inbred populations where roughly 200,000 and 25,000 sequence tags were mapped, respectively. An advantage in species like barley that lack a complete genome sequence is that a reference map need only be developed around the restriction sites, and this can be done in the process of sample genotyping. In such cases, the consensus of the read clusters across the sequence tagged sites becomes the reference. Alternatively, for kinship analyses in the absence of a reference genome, the sequence tags can simply be treated as dominant markers. Future application of GBS to breeding, conservation, and global species and population surveys may allow plant breeders to conduct genomic selection on a novel germplasm or species without first having to develop any prior molecular tools, or conservation biologists to determine population structure without prior knowledge of the genome or diversity in the species.read more
Citations
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Stacks: an analysis tool set for population genomics
Julian M. Catchen,Paul A. Hohenlohe,Paul A. Hohenlohe,Susan Bassham,Angel Amores,William A. Cresko +5 more
TL;DR: The expanded population genomics functions in Stacks will make it a useful tool to harness the newest generation of massively parallel genotyping data for ecological and evolutionary genetics.
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Genome-wide genetic marker discovery and genotyping using next-generation sequencing.
TL;DR: Best practices for several NGS methods for genome-wide genetic marker development and genotyping that use restriction enzyme digestion of target genomes to reduce the complexity of the target.
Journal ArticleDOI
Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme Genotyping-by-Sequencing Approach
Jesse Poland,Jesse Poland,Patrick J. Brown,Mark E. Sorrells,Jean-Luc Jannink,Jean-Luc Jannink +5 more
TL;DR: The GBS approach presented here provides a powerful method of developing high-density markers in species without a sequenced genome while providing valuable tools for anchoring and ordering physical maps and whole-genome shotgun sequence.
Journal ArticleDOI
Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array
Shichen Wang,Debbie Wong,Kerrie Forrest,Alexandra M. Allen,Shiaoman Chao,Bevan Emma Huang,Marco Maccaferri,Silvio Salvi,Sara Giulia Milner,Luigi Cattivelli,Anna M. Mastrangelo,Alex Whan,Stuart Stephen,Gary L A Barker,Ralf Wieseke,Joerg Plieske,Morten Lillemo,Diane E. Mather,Rudi Appels,Rudy Dolferus,Gina Brown-Guedira,Abraham B. Korol,Alina Akhunova,Catherine Feuillet,Catherine Feuillet,Jérôme Salse,Michele Morgante,Curtis J. Pozniak,Ming-Cheng Luo,Jan Dvorak,Matthew K. Morell,Jorge Dubcovsky,Jorge Dubcovsky,Martin W. Ganal,Roberto Tuberosa,Cindy Lawley,Ivan Mikoulitch,Colin Cavanagh,Keith J. Edwards,Matthew J. Hayden,Eduard Akhunov +40 more
TL;DR: The developed array and cluster identification algorithms provide an opportunity to infer detailed haplotype structure in polyploid wheat and will serve as an invaluable resource for diversity studies and investigating the genetic basis of trait variation in wheat.
Journal ArticleDOI
A physical, genetic and functional sequence assembly of the barley genome
Klaus F. X. Mayer,Robbie Waugh,Peter Langridge,Timothy J. Close,Roger P. Wise,Andreas Graner,Takashi Matsumoto,Kazuhiro Sato,Alan H. Schulman,Ruvini Ariyadasa,Daniela Schulte,Naser Poursarebani,Ruonan Zhou,Burkhard Steuernagel,Martin Mascher,Uwe Scholz,Bu-Jun Shi,Kavitha Madishetty,Jan T. Svensson,Prasanna R. Bhat,Matthew J. Moscou,Josh Resnik,Gary J. Muehlbauer,Pete E. Hedley,Hui Liu,Jenny Morris,Zeev Frenkel,Avraham Korol,Hélène Bergès,Stefan Taudien,Marius Felder,Marco Groth,Matthias Platzer,Axel Himmelbach,Stefano Lonardi,Denisa Duma,Matthew Alpert,Francesa Cordero,Francesa Cordero,Marco Beccuti,Gianfranco Ciardo,Yaqin Ma,Steve Wanamaker,Federica Cattonaro,Vera Vendramin,Simone Scalabrin,Slobodanka Radovic,Rod A. Wing,Michele Morgante,Thomas Nussbaumer,Heidrun Gundlach,Mihaela Martis,Jesse Poland,Matthias Pfeifer,Cédric Moisy,Jaakko Tanskanen,Andrea Zuccolo,Manuel Spannagl,Joanne Russell,Arnis Druka,David Marshall,Micha Bayer,David Swarbreck,Dharanya Sampath,Sarah Ayling,Melanie Febrer,Mario Caccamo,Tsuyoshi Tanaka,Steve Wannamaker,Thomas Schmutzer,John W. S. Brown,John W. S. Brown,Geoffrey B. Fincher,Nils Stein +73 more
TL;DR: An integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context and suggests that post-transcriptional processing forms an important regulatory layer.
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