Journal ArticleDOI
Role of Bacterial Intimin in Colonic Hyperplasia and Inflammation
Lisa M. Higgins,Gad Frankel,Ian F. Connerton,Nathalie S. Gonçalves,Gordon Dougan,Thomas T. MacDonald +5 more
TLDR
Mutation of cysteine-937 of intimin to alanine reduced costimulatory activity in vitro and prevented immunopathology in vivo and enables the bacteria to promote conditions that are favorable for increased microbial colonization.Abstract:
Enteropathogenic Escherichia coli (EPEC) cells adhere to gut epithelial cells through intimin α: the ligand for a bacterially derived epithelial transmembrane protein called the translocated intimin receptor. Citrobacter rodentium colonizes the mouse colon in a similar fashion and uses a different intimin: intimin β. Intimin α was found to costimulate submitogenic signals through the T cell receptor. Dead intimin β+ C. rodentium , intimin α–transfected C. rodentium or E. coli strain K12, and EPEC induced mucosal hyperplasia identical to that caused by C. rodentium live infection, as well as a massive T helper cell–type 1 immune response in the colonic mucosa. Mutation of cysteine-937 of intimin to alanine reduced costimulatory activity in vitro and prevented immunopathology in vivo. The mucosal changes elicited by C. rodentium were interferon-γ–dependent. Immunopathology induced by intimin enables the bacteria to promote conditions that are favorable for increased microbial colonization.read more
Citations
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Pathogenic Escherichia coli
TL;DR: Few microorganisms are as versatile as Escherichia coli; it can also be a highly versatile, and frequently deadly, pathogen.
Journal ArticleDOI
Clinical Aspects and Pathophysiology of Inflammatory Bowel Disease
TL;DR: The clinical manifestations and diagnostic features of IBD are delineated, and important recent advances in the understanding of the immune mediators of intestinal inflammation are summarized.
Journal ArticleDOI
Citrobacter rodentium of mice and man
TL;DR: The literature of C. rodentium is reviewed from its emergence in the mid‐1960s to the most contemporary reports of colonization, pathogenesis, transmission and immunity, providing an excellent in vivo model for A/E lesion forming pathogens.
Journal ArticleDOI
Intestinal colonization resistance
Trevor D. Lawley,Alan W. Walker +1 more
TL;DR: A holistic view that incorporates immunological and microbiological facets of the intestinal ecosystem should facilitate the development of immunomodulatory and microbe‐modulatory therapies that promote intestinal homeostasis and colonization resistance.
Journal ArticleDOI
Citrobacter rodentium: infection, inflammation and the microbiota
James W. Collins,Kristie M. Keeney,Valerie F. Crepin,Vijay A. K. Rathinam,Katherine A. Fitzgerald,B. Brett Finlay,Gad Frankel +6 more
TL;DR: Recent studies in which C. rodentium has been used to study mucosal immunology are discussed, including the deregulation of intestinal inflammatory responses during bacteria-induced colitis and the role of the intestinal microbiota in mediating resistance to colonization by enteric pathogens.
References
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Journal ArticleDOI
Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements
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Journal Article
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